Methods Of Producing Rpe Cells


Ontology type: sgo:Patent     


Patent Info

DATE

2014-06-12T00:00

AUTHORS

HOVATTA, OUTI , TRYGGVASON, KARL

ABSTRACT

The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno- free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "HOVATTA, OUTI", 
        "type": "Person"
      }, 
      {
        "name": "TRYGGVASON, KARL", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nbt1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030407065", 
          "https://doi.org/10.1038/nbt1177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030407065", 
          "https://doi.org/10.1038/nbt1177"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-06-12T00:00", 
    "description": "

The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno- free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.

", "id": "sg:patent.WO-2014087244-A2", "keywords": [ "RPE cell", "disclosure", "retinal pigment epithelium", "cell", "human embryonic stem cell", "plate", "laminin", "condition", "cell culture medium", "growth factor", "stem cell", "long time period", "retinal pigment epithelium cell" ], "name": "METHODS OF PRODUCING RPE CELLS", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.451737.3", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/WO-2014087244-A2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:13", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00790.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.WO-2014087244-A2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.WO-2014087244-A2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.WO-2014087244-A2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.WO-2014087244-A2'


 

This table displays all metadata directly associated to this object as RDF triples.

43 TRIPLES      15 PREDICATES      28 URIs      21 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.WO-2014087244-A2 schema:about anzsrc-for:2581
2 schema:author N42bd695f5922470ca27ee9bbdaacbb64
3 schema:citation sg:pub.10.1038/nbt1177
4 schema:datePublished 2014-06-12T00:00
5 schema:description <p>The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno- free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.</p>
6 schema:keywords RPE cell
7 cell
8 cell culture medium
9 condition
10 disclosure
11 growth factor
12 human embryonic stem cell
13 laminin
14 long time period
15 plate
16 retinal pigment epithelium
17 retinal pigment epithelium cell
18 stem cell
19 schema:name METHODS OF PRODUCING RPE CELLS
20 schema:recipient https://www.grid.ac/institutes/grid.451737.3
21 schema:sameAs https://app.dimensions.ai/details/patent/WO-2014087244-A2
22 schema:sdDatePublished 2019-04-18T10:13
23 schema:sdLicense https://scigraph.springernature.com/explorer/license/
24 schema:sdPublisher N1562e06712f24d8dab4791f8874e1754
25 sgo:license sg:explorer/license/
26 sgo:sdDataset patents
27 rdf:type sgo:Patent
28 N1562e06712f24d8dab4791f8874e1754 schema:name Springer Nature - SN SciGraph project
29 rdf:type schema:Organization
30 N42bd695f5922470ca27ee9bbdaacbb64 rdf:first Nf53fd0cb8f384ed69506bc5aea5a5ac0
31 rdf:rest N574b559600f34d47921a06cde9888560
32 N574b559600f34d47921a06cde9888560 rdf:first N850ba0f30cd94cb7b568d79a25e1fafd
33 rdf:rest rdf:nil
34 N850ba0f30cd94cb7b568d79a25e1fafd schema:name TRYGGVASON, KARL
35 rdf:type schema:Person
36 Nf53fd0cb8f384ed69506bc5aea5a5ac0 schema:name HOVATTA, OUTI
37 rdf:type schema:Person
38 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
39 rdf:type schema:DefinedTerm
40 sg:pub.10.1038/nbt1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030407065
41 https://doi.org/10.1038/nbt1177
42 rdf:type schema:CreativeWork
43 https://www.grid.ac/institutes/grid.451737.3 schema:Organization
 




Preview window. Press ESC to close (or click here)


...