Amplification Primers And Methods


Ontology type: sgo:Patent     


Patent Info

DATE

2013-06-27T00:00

AUTHORS

GRAY, Philip N. , ESHOO, MARK W.

ABSTRACT

The present invention provides methods, compositions, and kits for performing amplification (e.g., whole genome amplification) employing primers that have a 5' restriction site, a 3' random sequence (e.g., a random hexamer), and an identifiable barcode sequence. In certain embodiments, the amplification generates individual amplified sequenced that are ligated together to form concatamers containing at least two amplified sequences (e.g., not contiguous on the original target sequence) that are separated by the barcode sequences. In particular embodiments, a plurality of the concatamers are sequenced and aligned with an alignment algorithm that uses the barcode sequences to identify artificial junctions between amplified sequences. More... »

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