Methods Of Enhancing Genetic Diagnosis


Ontology type: sgo:Patent     


Patent Info

DATE

2013-06-06T00:00

AUTHORS

TRYGGVASON, KARL , TRYGGVASON, Kristian

ABSTRACT

The present disclosure relates to the use of laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. Methods of enhancing genetic diagnosis by expanding a single stem cell on laminin-521 prior to PCR amplification are disclosed herein. More... »

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