High Through-Put Analysis Of Transgene Borders


Ontology type: sgo:Patent     


Patent Info

DATE

2013-05-30T00:00

AUTHORS

CAO, ZEHUI , NOVAK, STEPHEN , SASTRY-DENT, Lakshmi , ZHOU, NING

ABSTRACT

A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "CAO, ZEHUI", 
        "type": "Person"
      }, 
      {
        "name": "NOVAK, STEPHEN", 
        "type": "Person"
      }, 
      {
        "name": "SASTRY-DENT, Lakshmi", 
        "type": "Person"
      }, 
      {
        "name": "ZHOU, NING", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02772830", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013512451", 
          "https://doi.org/10.1007/bf02772830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/18.10.3095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040264608"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-05-30T00:00", 
    "description": "

A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.

", "id": "sg:patent.WO-2013078318-A1", "keywords": [ "transgene", "method", "chromosomal DNA", "flanking region", "chromosome", "wherein", "isolation", "digestion", "genomic DNA", "restriction enzyme", "ligation", "adapter", "primer extension", "streptavidin", "PCR amplification", "DNA Sequence Analysis" ], "name": "HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.418574.b", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/WO-2013078318-A1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:11", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00541.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.WO-2013078318-A1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.WO-2013078318-A1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.WO-2013078318-A1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.WO-2013078318-A1'


 

This table displays all metadata directly associated to this object as RDF triples.

57 TRIPLES      15 PREDICATES      32 URIs      24 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.WO-2013078318-A1 schema:about anzsrc-for:2620
2 schema:author Ne59d2ef15053450e8fcc7f023f1b196e
3 schema:citation sg:pub.10.1007/bf02772830
4 https://doi.org/10.1093/nar/18.10.3095
5 schema:datePublished 2013-05-30T00:00
6 schema:description <p>A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.</p>
7 schema:keywords DNA Sequence Analysis
8 PCR amplification
9 adapter
10 chromosomal DNA
11 chromosome
12 digestion
13 flanking region
14 genomic DNA
15 isolation
16 ligation
17 method
18 primer extension
19 restriction enzyme
20 streptavidin
21 transgene
22 wherein
23 schema:name HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS
24 schema:recipient https://www.grid.ac/institutes/grid.418574.b
25 schema:sameAs https://app.dimensions.ai/details/patent/WO-2013078318-A1
26 schema:sdDatePublished 2019-04-18T10:11
27 schema:sdLicense https://scigraph.springernature.com/explorer/license/
28 schema:sdPublisher N11cbe279d9a242a9b594c9d23b935173
29 sgo:license sg:explorer/license/
30 sgo:sdDataset patents
31 rdf:type sgo:Patent
32 N0ec03fd9ea2f4a37bf11d36225bb5cb0 schema:name ZHOU, NING
33 rdf:type schema:Person
34 N11cbe279d9a242a9b594c9d23b935173 schema:name Springer Nature - SN SciGraph project
35 rdf:type schema:Organization
36 N33bd02cb353c433a807f811eaf451d64 rdf:first N864d009ae2424e2f822a9c5ad23d60ed
37 rdf:rest N811ccad0615143e4a9b67f5ad489f7c9
38 N42a11ed30ead469ba66d92680a1de874 schema:name NOVAK, STEPHEN
39 rdf:type schema:Person
40 N5c984fc7298540f2946ee8df34cff925 rdf:first N42a11ed30ead469ba66d92680a1de874
41 rdf:rest N33bd02cb353c433a807f811eaf451d64
42 N811ccad0615143e4a9b67f5ad489f7c9 rdf:first N0ec03fd9ea2f4a37bf11d36225bb5cb0
43 rdf:rest rdf:nil
44 N8520a13d902347689880f0e1fa7f528e schema:name CAO, ZEHUI
45 rdf:type schema:Person
46 N864d009ae2424e2f822a9c5ad23d60ed schema:name SASTRY-DENT, Lakshmi
47 rdf:type schema:Person
48 Ne59d2ef15053450e8fcc7f023f1b196e rdf:first N8520a13d902347689880f0e1fa7f528e
49 rdf:rest N5c984fc7298540f2946ee8df34cff925
50 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
51 rdf:type schema:DefinedTerm
52 sg:pub.10.1007/bf02772830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013512451
53 https://doi.org/10.1007/bf02772830
54 rdf:type schema:CreativeWork
55 https://doi.org/10.1093/nar/18.10.3095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040264608
56 rdf:type schema:CreativeWork
57 https://www.grid.ac/institutes/grid.418574.b schema:Organization
 




Preview window. Press ESC to close (or click here)


...