Cell Culture Medium


Ontology type: sgo:Patent     


Patent Info

DATE

2012-06-21T00:00

AUTHORS

TRYGGVASON, KARL , RODIN, SERGEY

ABSTRACT

The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "TRYGGVASON, KARL", 
        "type": "Person"
      }, 
      {
        "name": "RODIN, SERGEY", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m111228200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012337970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2005.03.058", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021906730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2005.03.058", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021906730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030407065", 
          "https://doi.org/10.1038/nbt1177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030407065", 
          "https://doi.org/10.1038/nbt1177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2005-0247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036210003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2005-0247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036210003"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-06-21T00:00", 
    "description": "

The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

", "id": "sg:patent.WO-2012080844-A1", "keywords": [ "cell culture medium", "disclosure", "laminin", "method", "host cell", "composition", "stem cell", "pluripotency", "cell survival", "human embryonic stem cell", "plate", "absence", "inhibitor", "feeder cell", "embryonic stem cell", "single cell suspension", "fibroblast growth factor" ], "name": "CELL CULTURE MEDIUM", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.451737.3", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/WO-2012080844-A1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:09", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00357.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.WO-2012080844-A1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.WO-2012080844-A1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.WO-2012080844-A1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.WO-2012080844-A1'


 

This table displays all metadata directly associated to this object as RDF triples.

56 TRIPLES      15 PREDICATES      35 URIs      25 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.WO-2012080844-A1 schema:about anzsrc-for:2581
2 schema:author Nb81f3ed3c8994dc887bbb1d6658b5c3f
3 schema:citation sg:pub.10.1038/nbt1177
4 https://doi.org/10.1016/j.bbrc.2005.03.058
5 https://doi.org/10.1074/jbc.m111228200
6 https://doi.org/10.1634/stemcells.2005-0247
7 schema:datePublished 2012-06-21T00:00
8 schema:description <p>The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.</p>
9 schema:keywords absence
10 cell culture medium
11 cell survival
12 composition
13 disclosure
14 embryonic stem cell
15 feeder cell
16 fibroblast growth factor
17 host cell
18 human embryonic stem cell
19 inhibitor
20 laminin
21 method
22 plate
23 pluripotency
24 single cell suspension
25 stem cell
26 schema:name CELL CULTURE MEDIUM
27 schema:recipient https://www.grid.ac/institutes/grid.451737.3
28 schema:sameAs https://app.dimensions.ai/details/patent/WO-2012080844-A1
29 schema:sdDatePublished 2019-04-18T10:09
30 schema:sdLicense https://scigraph.springernature.com/explorer/license/
31 schema:sdPublisher N178432175b1d4cf0a556c54ac3a8560a
32 sgo:license sg:explorer/license/
33 sgo:sdDataset patents
34 rdf:type sgo:Patent
35 N178432175b1d4cf0a556c54ac3a8560a schema:name Springer Nature - SN SciGraph project
36 rdf:type schema:Organization
37 N3ecc7e17b09c4d1eb5d8373df8dd4be0 rdf:first N690ca7643c6941b4987d51f66540fee0
38 rdf:rest rdf:nil
39 N690ca7643c6941b4987d51f66540fee0 schema:name RODIN, SERGEY
40 rdf:type schema:Person
41 Nab23851644d34c838e667d15faa63eaf schema:name TRYGGVASON, KARL
42 rdf:type schema:Person
43 Nb81f3ed3c8994dc887bbb1d6658b5c3f rdf:first Nab23851644d34c838e667d15faa63eaf
44 rdf:rest N3ecc7e17b09c4d1eb5d8373df8dd4be0
45 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
46 rdf:type schema:DefinedTerm
47 sg:pub.10.1038/nbt1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030407065
48 https://doi.org/10.1038/nbt1177
49 rdf:type schema:CreativeWork
50 https://doi.org/10.1016/j.bbrc.2005.03.058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021906730
51 rdf:type schema:CreativeWork
52 https://doi.org/10.1074/jbc.m111228200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012337970
53 rdf:type schema:CreativeWork
54 https://doi.org/10.1634/stemcells.2005-0247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036210003
55 rdf:type schema:CreativeWork
56 https://www.grid.ac/institutes/grid.451737.3 schema:Organization
 




Preview window. Press ESC to close (or click here)


...