Dna Library And Preparation Method Thereof, And Method And Device For Detecting Snps


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

杜野 , 赵美茹 , 陈颖 , 武靖华 , 田埂 , 王俊

ABSTRACT

Provided are a DNA library and preparation method thereof, a method for determining DNA sequence information, a device and a kit for detecting SNPs, and a method for genotyping. The method for preparing the DNA library comprises the following steps: digesting the genomic DNA of a sample with a restriction endonuclease to obtain enzyme-digested products, wherein the restriction endonuclease comprises at least one selected from MBo II and Tsp 45I; separating the enzyme-digested products to obtain DNA fragments of 100 bp to 1,000 bp in length; subjecting the DNA fragments to terminal repair to obtain terminally repaired DNA fragments; adding the base A to the termini of the terminally repaired DNA fragments to obtain DNA fragments with terminal base A; and linking the DNA fragments with terminal base A to linkers for sequencing to obtain the DNA library. More... »

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