Stem Cell Defined Media For Xeno-Free And Feeder Free Conditions And Uses Thereof


Ontology type: sgo:Patent     


Patent Info

DATE

2012-04-19T00:00

AUTHORS

DOS REIS CASTILHO, LEDA , MARINHO, PAULO ANDRE, NOBREGA , MUOTRI, ALYSSON , REHEN, STEVENS, KASTRUP

ABSTRACT

The invention provides a defined low protein culture medium for maintaining cells in an undifferentiated state, the medium comprising: a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, a combination of growth factors selected from the group consisting of FGF-2 protein, an IGF-1 protein or insulin, a Transferrin protein, and a TGF beta 1 protein, wherein the medium is essentially feeder-free, essentially xeno-free, essentially free of beta-mercaptoethanol, and essentially free of animal-derived or human-derived proteins. More... »

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