Method And Kit For Dna Extraction From Vitis Vinifera L. And For Amplification And Detection Of Grapevine Varieties Or Cultivars ...


Ontology type: sgo:Patent     


Patent Info

DATE

2011-06-09T00:00

AUTHORS

MARTINS LOPES, PAULA FILOMENA , GONÇALVES PEREIRA, Maria Leonor , DE PINHO GUEDES PINTO, HENRIQUE

ABSTRACT

The present invention describes a method for extracting DNA from Vitis vinifera L. comprising the following stages: a. precipitating the wine or must samples with isopropanol or 2 propanol, extracting and collecting the pellet; b. incubating the sample resulting from the previous stage with an extraction buffer comprising: • preferably a membrane solubilizer; • one or more anti-oxidizing agents; • a chelant of divalent cations, Mg+2 and/or Ca+2; c. performing one or more extractions on the sample resulting from the previous stage using chloroform and/or phenol; d. performing one or more periods of precipitation and incubation with RNases; e. detecting the grapevine varieties or cultivars. The invention also describes a DNA extraction kit for wine or must samples comprising a solution that contains said extraction buffer, and the extraction is conducted according to the described method. The method and kit can be implemented as a traceability system for ascertaining the origin of the product, detecting counterfeits and mislabelling, and represents an advantageous solution in that it allows grapevine DNA extraction from monovarietal, varietal and commercial musts and wines, thereby making the detection and quantification of different grapevine varieties in musts and wines possible. More... »

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