Use Of Double Stranded Rna To Increase The Efficiency Of Targeted Gene Alteration In Plant Protoplasts


Ontology type: sgo:Patent     


Patent Info

DATE

2010-07-01T00:00

AUTHORS

BUNDOCK, PAUL

ABSTRACT

Method for targeted gene alteration in protoplasts of plant cells comprising the steps of transiently transfecting the protoplasts with a dsRNA that preferably targets plant MMR mRNA; and a mutagenic nucleobase. The transfection may be simultaneously or subsequently and the gene can be any gene functional in the mismatch repair system. More... »

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