Dna Sequencing Method And System


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

SHAO, ZHIFENG , SHENG, SITONG

ABSTRACT

The invention provides a DNA sequencing method and system. The said DNA sequencing method comprises the steps below: A. The DNA is treated into a variety of DNA fragments, and prepared into a variety of DNA signatures; B. Each of DNA signatures is amplified and treated into the single strand; C. Each of DNA signatures is sequenced by using the anchor primers which can operationally link with the DNA signature and have at least one degenerate base, and therefore the sequencing signal is generated; D. According to the sequencing signal, determine the sequence of each of DNA fragments. The said DNA sequencing system comprises a manufacture unit, an amplification unit, a sequencing reaction unit and a signal treatment unit of short signatures. During the DNA sequencing, the DNA signature is sequenced by using the anchor primers which have at least one degenerate base. Consequently, the invention method prolongs the length range capable of direct-sequencing the short DNA signature. The invention can sequence the shorter and the longer DNA signatures, and broaden the application range of DNA sequencing. More... »

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