Expression Vectors For Concentrating A Recombinantly Produced Protein In Different Cell Compartments


Ontology type: sgo:Patent     


Patent Info

DATE

2001-10-04T00:00

AUTHORS

Düring, Klaus

ABSTRACT

The invention relates to an expression vector (A) characterized by containing at least two copies of a gene encoding a desired protein in functional combination with a promoter, or to a composition (B) comprising at least two expression vectors containing at least one copy of a gene encoding a desired protein in functional combination with a promoter. The individual gene copies are characterized in that (a) they encode the desired protein (I) as a fusion protein with a desired localization signal (II); (b) the (I) encoding portion of the individual gene copies being substantially identical, the (II) encoding portion however being different. Once the expression vector (A) or the composition (B) is introduced into a host, the desired protein is transported to different compartments of the host. The invention further relates to a composition (C) comprising at least one expression vector localized in the plastids and one expression vector localized in the mitochondria, both containing at least one copy of a gene encoding the desired protein functionally linked with a promoter. The individual expression vectors are characterized in that the individual gene copies are substantially identical and the desired protein is expressed in the plastids or the mitochondria of the host. The inventive expression vectors/compositions are used in methods that allow to increase the production rate of recombinant proteins. More... »

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