Process For Detecting And Quantifying Nucleic Acid Molecules


Ontology type: sgo:Patent     


Patent Info

DATE

1997-07-17T00:00

AUTHORS

BERTLING, WOLF

ABSTRACT

The invention relates to a process for detecting and quantifying nucleic acid (=NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: a) heteroduplexes are formed in which nucleotides which are unpaired or, according to Watson and Crick, incorrectly paired are chemically reactive with a carbon di-imide compound; b) the single or double-stranded ribonucleic acid (=RNA) molecules or single-stranded deoxyribonucleic acid (=DNA) molecules contained in population (I) are transferred into a population (II) of double-stranded DNA molecules; c) the population (II) of double-stranded DNA molecules are subjected to denaturation and subsequent renaturation, to produce a mixture of homo and heteroduplex DNA molecules. To make it possible to quantify the NA molecules detected, it is proposed that a purification stage take place between steps a) and b) and between steps b) and c). More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "BERTLING, WOLF", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0027-5107(93)90208-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009816385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.24.5082", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014309292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng0295-177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015620800", 
          "https://doi.org/10.1038/ng0295-177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/18.13.3933", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033687600"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-07-17T00:00", 
    "description": "

The invention relates to a process for detecting and quantifying nucleic acid (=NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: a) heteroduplexes are formed in which nucleotides which are unpaired or, according to Watson and Crick, incorrectly paired are chemically reactive with a carbon di-imide compound; b) the single or double-stranded ribonucleic acid (=RNA) molecules or single-stranded deoxyribonucleic acid (=DNA) molecules contained in population (I) are transferred into a population (II) of double-stranded DNA molecules; c) the population (II) of double-stranded DNA molecules are subjected to denaturation and subsequent renaturation, to produce a mixture of homo and heteroduplex DNA molecules. To make it possible to quantify the NA molecules detected, it is proposed that a purification stage take place between steps a) and b) and between steps b) and c).

", "id": "sg:patent.WO-1997025441-A2", "keywords": [ "nucleic acid molecule", "invention", "nucleic acid", "molecule", "population", "different sequence", "heteroduplexes", "nucleotide", "Watson", "compound", "ribonucleic acids", "acid", "DNA molecule", "denaturation", "renaturation", "mixture", "purification" ], "name": "PROCESS FOR DETECTING AND QUANTIFYING NUCLEIC ACID MOLECULES", "sameAs": [ "https://app.dimensions.ai/details/patent/WO-1997025441-A2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:30", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03431.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.WO-1997025441-A2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.WO-1997025441-A2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.WO-1997025441-A2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.WO-1997025441-A2'


 

This table displays all metadata directly associated to this object as RDF triples.

50 TRIPLES      14 PREDICATES      34 URIs      25 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.WO-1997025441-A2 schema:about anzsrc-for:2620
2 schema:author N157e487a7a824a8597812eecf81c59ea
3 schema:citation sg:pub.10.1038/ng0295-177
4 https://doi.org/10.1016/0027-5107(93)90208-w
5 https://doi.org/10.1093/nar/18.13.3933
6 https://doi.org/10.1093/nar/23.24.5082
7 schema:datePublished 1997-07-17T00:00
8 schema:description <p>The invention relates to a process for detecting and quantifying nucleic acid (=NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: a) heteroduplexes are formed in which nucleotides which are unpaired or, according to Watson and Crick, incorrectly paired are chemically reactive with a carbon di-imide compound; b) the single or double-stranded ribonucleic acid (=RNA) molecules or single-stranded deoxyribonucleic acid (=DNA) molecules contained in population (I) are transferred into a population (II) of double-stranded DNA molecules; c) the population (II) of double-stranded DNA molecules are subjected to denaturation and subsequent renaturation, to produce a mixture of homo and heteroduplex DNA molecules. To make it possible to quantify the NA molecules detected, it is proposed that a purification stage take place between steps a) and b) and between steps b) and c).</p>
9 schema:keywords DNA molecule
10 Watson
11 acid
12 compound
13 denaturation
14 different sequence
15 heteroduplexes
16 invention
17 mixture
18 molecule
19 nucleic acid
20 nucleic acid molecule
21 nucleotide
22 population
23 purification
24 renaturation
25 ribonucleic acids
26 schema:name PROCESS FOR DETECTING AND QUANTIFYING NUCLEIC ACID MOLECULES
27 schema:sameAs https://app.dimensions.ai/details/patent/WO-1997025441-A2
28 schema:sdDatePublished 2019-04-18T10:30
29 schema:sdLicense https://scigraph.springernature.com/explorer/license/
30 schema:sdPublisher N839b469be16d461998317d0c07fef548
31 sgo:license sg:explorer/license/
32 sgo:sdDataset patents
33 rdf:type sgo:Patent
34 N157e487a7a824a8597812eecf81c59ea rdf:first N5b6ab8fedd6146e6950779853c344713
35 rdf:rest rdf:nil
36 N5b6ab8fedd6146e6950779853c344713 schema:name BERTLING, WOLF
37 rdf:type schema:Person
38 N839b469be16d461998317d0c07fef548 schema:name Springer Nature - SN SciGraph project
39 rdf:type schema:Organization
40 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
41 rdf:type schema:DefinedTerm
42 sg:pub.10.1038/ng0295-177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015620800
43 https://doi.org/10.1038/ng0295-177
44 rdf:type schema:CreativeWork
45 https://doi.org/10.1016/0027-5107(93)90208-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1009816385
46 rdf:type schema:CreativeWork
47 https://doi.org/10.1093/nar/18.13.3933 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033687600
48 rdf:type schema:CreativeWork
49 https://doi.org/10.1093/nar/23.24.5082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014309292
50 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...