The Reduction Of Non-Specific Amplification During In Vitro Nucleic Acid Amplification Using Modified Nucleic Acid Bases


Ontology type: sgo:Patent     


Patent Info

DATE

1992-05-14T00:00

AUTHORS

GELFAND DAVID H , KWOK SHIRLEY Y , SNINSKY JOHN J

ABSTRACT

Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/3053", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "GELFAND DAVID H", 
        "type": "Person"
      }, 
      {
        "name": "KWOK SHIRLEY Y", 
        "type": "Person"
      }, 
      {
        "name": "SNINSKY JOHN J", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0888-7543(89)90280-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004053545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1088-1197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011078389", 
          "https://doi.org/10.1038/nbt1088-1197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.5.1874", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019856430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.2.488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026991187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90145-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036212402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90145-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036212402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00288a026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055168789"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1992-05-14T00:00", 
    "description": "

Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.

", "id": "sg:patent.WO-1992001814-A3", "keywords": [ "nucleic acid amplification", "improved method", "nucleic acid", "amplification", "contamination", "amplified product", "method", "introduction", "nucleotide base", "reaction product", "product", "glycosylase", "chemical mean", "template" ], "name": "THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.418424.f", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/WO-1992001814-A3" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:30", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03411.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.WO-1992001814-A3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.WO-1992001814-A3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.WO-1992001814-A3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.WO-1992001814-A3'


 

This table displays all metadata directly associated to this object as RDF triples.

63 TRIPLES      15 PREDICATES      34 URIs      22 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.WO-1992001814-A3 schema:about anzsrc-for:3053
2 schema:author N4dadb6a6adaf471e93365974feb143d5
3 schema:citation sg:pub.10.1038/nbt1088-1197
4 https://doi.org/10.1016/0378-1119(90)90145-h
5 https://doi.org/10.1016/0888-7543(89)90280-2
6 https://doi.org/10.1021/bi00288a026
7 https://doi.org/10.1073/pnas.82.2.488
8 https://doi.org/10.1073/pnas.87.5.1874
9 schema:datePublished 1992-05-14T00:00
10 schema:description <p>Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.</p>
11 schema:keywords amplification
12 amplified product
13 chemical mean
14 contamination
15 glycosylase
16 improved method
17 introduction
18 method
19 nucleic acid
20 nucleic acid amplification
21 nucleotide base
22 product
23 reaction product
24 template
25 schema:name THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING IN VITRO NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES
26 schema:recipient https://www.grid.ac/institutes/grid.418424.f
27 schema:sameAs https://app.dimensions.ai/details/patent/WO-1992001814-A3
28 schema:sdDatePublished 2019-04-18T10:30
29 schema:sdLicense https://scigraph.springernature.com/explorer/license/
30 schema:sdPublisher Nba8a11be8186484289b604d4609be230
31 sgo:license sg:explorer/license/
32 sgo:sdDataset patents
33 rdf:type sgo:Patent
34 N18f5ecf2d17d4c729376ac5fff5300a4 schema:name GELFAND DAVID H
35 rdf:type schema:Person
36 N277927f6f4214bc09204a34e359f4c63 schema:name SNINSKY JOHN J
37 rdf:type schema:Person
38 N4dadb6a6adaf471e93365974feb143d5 rdf:first N18f5ecf2d17d4c729376ac5fff5300a4
39 rdf:rest N5065a57b59c94c8c82fc3ce46469afc9
40 N5065a57b59c94c8c82fc3ce46469afc9 rdf:first Neb5bb2bd47324e25a98a5f0d91e7314a
41 rdf:rest N88604437828f47ddb5602c02ba737519
42 N88604437828f47ddb5602c02ba737519 rdf:first N277927f6f4214bc09204a34e359f4c63
43 rdf:rest rdf:nil
44 Nba8a11be8186484289b604d4609be230 schema:name Springer Nature - SN SciGraph project
45 rdf:type schema:Organization
46 Neb5bb2bd47324e25a98a5f0d91e7314a schema:name KWOK SHIRLEY Y
47 rdf:type schema:Person
48 anzsrc-for:3053 schema:inDefinedTermSet anzsrc-for:
49 rdf:type schema:DefinedTerm
50 sg:pub.10.1038/nbt1088-1197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011078389
51 https://doi.org/10.1038/nbt1088-1197
52 rdf:type schema:CreativeWork
53 https://doi.org/10.1016/0378-1119(90)90145-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1036212402
54 rdf:type schema:CreativeWork
55 https://doi.org/10.1016/0888-7543(89)90280-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004053545
56 rdf:type schema:CreativeWork
57 https://doi.org/10.1021/bi00288a026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055168789
58 rdf:type schema:CreativeWork
59 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
60 rdf:type schema:CreativeWork
61 https://doi.org/10.1073/pnas.87.5.1874 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019856430
62 rdf:type schema:CreativeWork
63 https://www.grid.ac/institutes/grid.418424.f schema:Organization
 




Preview window. Press ESC to close (or click here)


...