The Reduction Of Non-Specific Amplification During In Vitro Nucleic Acid Amplification Using Modified Nucleic Acid Bases


Ontology type: sgo:Patent     


Patent Info

DATE

1992-02-06T00:00

AUTHORS

SNINSKY, JOHN, J. , GELFAND, DAVID, H. , KWOK, SHIRLEY, Y.

ABSTRACT

Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications. More... »

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