Procaryotic Xylose Isomerase Muteins And Method To Increase Protein Stability


Ontology type: sgo:Patent     


Patent Info

DATE

1989-02-23T00:00

AUTHORS

DRUMMOND, ROBERT, J. , BLOCH, WILL , MATTHEWS, BRIAN, W. , TOY, PAMELA, L. , NICHOLSON, HENRY, H.

ABSTRACT

Xylose isomerase (XI) muteins useful in the conversion of glucose to fructose or xylose to xylulose are obtained in usable amounts by protein structural and recombinant DNA methods, including x-ray crystallography, cloning, computer graphic modeling and site-directed mutagenesis and expression of the bacterial DNA sequences encoding native procaryotic xylose isomerase. These native sequences are altered to encode the xylose isomerase muteins having improved catalytic function and/or thermostability, and/or a lowered pH optimum. A method for predicting protein-stabilizing amino acid substitutions is also provided. More... »

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