Detection of elevated levels of phosphorylated MCM and method of increasing MCM phosphorylation capacity


Ontology type: sgo:Patent     


Patent Info

DATE

2018-05-22T00:00

AUTHORS

Daniel L. Kaplan , Irina Bruck

ABSTRACT

Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/3142", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Daniel L. Kaplan", 
        "type": "Person"
      }, 
      {
        "name": "Irina Bruck", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1083/jcb.107.5.1623", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002229533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002251969", 
          "https://doi.org/10.1038/nrc1569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002251969", 
          "https://doi.org/10.1038/nrc1569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/gut.46.6.746", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002836009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11920", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003048912", 
          "https://doi.org/10.1038/nature11920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003172621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.11.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003604838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2010.11.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003604838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00412-014-0489-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004223733", 
          "https://doi.org/10.1007/s00412-014-0489-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceb.2010.07.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004517774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201102003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005871957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.12.049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006869070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.12.049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006869070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.12.049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006869070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14285", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007234009", 
          "https://doi.org/10.1038/nature14285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(02)08739-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009511777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/20.8.2097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010219249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1463-1318.2004.00567.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010285696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6601234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011203305", 
          "https://doi.org/10.1038/sj.bjc.6601234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6601234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011203305", 
          "https://doi.org/10.1038/sj.bjc.6601234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0560.1998.tb01747.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011730055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.246827.114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012013105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkr371", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012228174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc2820", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012705094", 
          "https://doi.org/10.1038/nrc2820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc2820", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012705094", 
          "https://doi.org/10.1038/nrc2820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1444", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013548862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.10.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014449249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.10.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014449249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.039123", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014503129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/02688690120114174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015995086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.218305", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017810177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.07.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022179585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.07.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022179585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2011.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022627276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2011.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022627276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08647", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022812644", 
          "https://doi.org/10.1038/nature08647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08647", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022812644", 
          "https://doi.org/10.1038/nature08647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m608935200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026939157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2990.2005.00618.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026965256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2990.2005.00618.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026965256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m114.608232", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027231397"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027906138", 
          "https://doi.org/10.1038/nrc1548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027906138", 
          "https://doi.org/10.1038/nrc1548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.11.24.3365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028548738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1509608112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029082981"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2011.404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030400958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.226332", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030636449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1933010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035610853"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.68.1.109-131.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035970361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.10.3262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036404102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq1222", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036541118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1883410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036815090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1593/neo.08216", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037593382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.21698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039499151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-4652(200003)182:3<311::aid-jcp1>3.0.co;2-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040757424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.242404.114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041205054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000078673", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042013792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05465", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042319022", 
          "https://doi.org/10.1038/nature05465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05465", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042319022", 
          "https://doi.org/10.1038/nature05465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2008.05.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042577158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2008.05.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042577158"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m113.532085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042604111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0604990103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043501647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jso.10287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043593285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-0714.2003.00116.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043798234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0911500106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044523994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.06.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044927218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.06.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044927218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0302-2838(02)00580-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045619954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0302-2838(02)00580-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045619954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m115.640383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047130932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa033403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047425758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.457807", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049848236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1759609", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049980699"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-04-1776", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050314186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m512921200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051278565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.0305-1846.2001.00333.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052756636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.07.045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052894008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.07.045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052894008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m115.688424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058214050"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2003.04.121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064203544"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2001.19.22.4259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074954341"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-05-22T00:00", 
    "description": "

Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells.

", "id": "sg:patent.US-9977026-B2", "keywords": [ "elevated level", "MCM", "method", "phosphorylation", "novel post-translational modification", "Mcm2", "wherein", "residue", "human cancer", "Colorectal Neoplasm", "early cancer", "high sensitivity", "Sensitivity and Specificity", "modification", "yeast", "DNA replication", "cell", "embodiment", "invention", "antibody", "human", "colon cancer cell line", "cancer cell line", "normal cell" ], "name": "Detection of elevated levels of phosphorylated MCM and method of increasing MCM phosphorylation capacity", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.255986.5", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-9977026-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:22", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_01752.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-9977026-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-9977026-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-9977026-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-9977026-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

267 TRIPLES      15 PREDICATES      107 URIs      32 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-9977026-B2 schema:about anzsrc-for:2581
2 anzsrc-for:3142
3 schema:author N2c5aa1bf61a84e11b46f79937d24a782
4 schema:citation sg:pub.10.1007/s00412-014-0489-2
5 sg:pub.10.1038/nature05432
6 sg:pub.10.1038/nature05465
7 sg:pub.10.1038/nature08647
8 sg:pub.10.1038/nature11920
9 sg:pub.10.1038/nature14285
10 sg:pub.10.1038/nrc1548
11 sg:pub.10.1038/nrc1569
12 sg:pub.10.1038/nrc2820
13 sg:pub.10.1038/sj.bjc.6601234
14 https://doi.org/10.1002/(sici)1097-4652(200003)182:3<311::aid-jcp1>3.0.co;2-9
15 https://doi.org/10.1002/jcb.21698
16 https://doi.org/10.1002/jso.10287
17 https://doi.org/10.1002/path.1444
18 https://doi.org/10.1016/j.ceb.2010.07.015
19 https://doi.org/10.1016/j.cell.2009.10.015
20 https://doi.org/10.1016/j.cell.2009.12.049
21 https://doi.org/10.1016/j.cell.2011.06.012
22 https://doi.org/10.1016/j.cell.2011.07.045
23 https://doi.org/10.1016/j.cub.2011.11.038
24 https://doi.org/10.1016/j.molcel.2006.07.033
25 https://doi.org/10.1016/j.molcel.2008.05.020
26 https://doi.org/10.1016/j.molcel.2009.12.030
27 https://doi.org/10.1016/j.molcel.2010.11.027
28 https://doi.org/10.1016/s0140-6736(02)08739-1
29 https://doi.org/10.1016/s0302-2838(02)00580-8
30 https://doi.org/10.1034/j.1600-0714.2003.00116.x
31 https://doi.org/10.1038/emboj.2011.404
32 https://doi.org/10.1038/sj.emboj.7601063
33 https://doi.org/10.1038/sj.emboj.7601075
34 https://doi.org/10.1046/j.0305-1846.2001.00333.x
35 https://doi.org/10.1056/nejmoa033403
36 https://doi.org/10.1073/pnas.0604990103
37 https://doi.org/10.1073/pnas.0911500106
38 https://doi.org/10.1073/pnas.1509608112
39 https://doi.org/10.1073/pnas.82.10.3262
40 https://doi.org/10.1074/jbc.m109.039123
41 https://doi.org/10.1074/jbc.m111.218305
42 https://doi.org/10.1074/jbc.m111.226332
43 https://doi.org/10.1074/jbc.m113.532085
44 https://doi.org/10.1074/jbc.m114.608232
45 https://doi.org/10.1074/jbc.m115.640383
46 https://doi.org/10.1074/jbc.m115.688424
47 https://doi.org/10.1074/jbc.m512921200
48 https://doi.org/10.1074/jbc.m608935200
49 https://doi.org/10.1080/02688690120114174
50 https://doi.org/10.1083/jcb.107.5.1623
51 https://doi.org/10.1083/jcb.201102003
52 https://doi.org/10.1093/emboj/20.8.2097
53 https://doi.org/10.1093/nar/gkq1222
54 https://doi.org/10.1093/nar/gkr371
55 https://doi.org/10.1101/gad.11.24.3365
56 https://doi.org/10.1101/gad.1759609
57 https://doi.org/10.1101/gad.1883410
58 https://doi.org/10.1101/gad.1933010
59 https://doi.org/10.1101/gad.242404.114
60 https://doi.org/10.1101/gad.246827.114
61 https://doi.org/10.1101/gad.457807
62 https://doi.org/10.1111/j.1365-2990.2005.00618.x
63 https://doi.org/10.1111/j.1463-1318.2004.00567.x
64 https://doi.org/10.1111/j.1600-0560.1998.tb01747.x
65 https://doi.org/10.1128/mmbr.68.1.109-131.2004
66 https://doi.org/10.1136/gut.46.6.746
67 https://doi.org/10.1158/1078-0432.ccr-04-1776
68 https://doi.org/10.1159/000078673
69 https://doi.org/10.1200/jco.2001.19.22.4259
70 https://doi.org/10.1200/jco.2003.04.121
71 https://doi.org/10.1593/neo.08216
72 schema:datePublished 2018-05-22T00:00
73 schema:description <p id="p-0001" num="0000">Identified herein is a novel post-translational modification of Mcm2, wherein the residue of serines 53 and 108 are phosphorylated. DDK phosphorylates Mcm2 at serines 53 and 108 when stimulated by Treslin. DDK is overexpressed in many human cancers, including colorectal cancer, suggesting that monitoring the phosphorylation of Mcm2 at serines 53 and 108 may detect early cancer with high sensitivity and specificity. It was also found that the homologous modification in budding yeast is required for DNA replication, and the modification occurs in cells during active DNA replication only. In an embodiment, the current invention is an antibody specific for human Mcm2 that is phosphorylated at serines 53 and 108. This antibody was found to be overexpressed in colon cancer cell line HCT 116, as well as other cancer cell lines, compared to normal cells.</p>
74 schema:keywords Colorectal Neoplasm
75 DNA replication
76 MCM
77 Mcm2
78 Sensitivity and Specificity
79 antibody
80 cancer cell line
81 cell
82 colon cancer cell line
83 early cancer
84 elevated level
85 embodiment
86 high sensitivity
87 human
88 human cancer
89 invention
90 method
91 modification
92 normal cell
93 novel post-translational modification
94 phosphorylation
95 residue
96 wherein
97 yeast
98 schema:name Detection of elevated levels of phosphorylated MCM and method of increasing MCM phosphorylation capacity
99 schema:recipient https://www.grid.ac/institutes/grid.255986.5
100 schema:sameAs https://app.dimensions.ai/details/patent/US-9977026-B2
101 schema:sdDatePublished 2019-04-18T10:22
102 schema:sdLicense https://scigraph.springernature.com/explorer/license/
103 schema:sdPublisher N0772c3b854a44271adb15da9e961d2dc
104 sgo:license sg:explorer/license/
105 sgo:sdDataset patents
106 rdf:type sgo:Patent
107 N0772c3b854a44271adb15da9e961d2dc schema:name Springer Nature - SN SciGraph project
108 rdf:type schema:Organization
109 N2c5aa1bf61a84e11b46f79937d24a782 rdf:first Ncf32337a9f504831a726ddb33721005d
110 rdf:rest N8efea0e41c384428bfa088a063fed6ed
111 N450a23fc1fe3407cb00b65d107d000a9 schema:name Irina Bruck
112 rdf:type schema:Person
113 N8efea0e41c384428bfa088a063fed6ed rdf:first N450a23fc1fe3407cb00b65d107d000a9
114 rdf:rest rdf:nil
115 Ncf32337a9f504831a726ddb33721005d schema:name Daniel L. Kaplan
116 rdf:type schema:Person
117 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
118 rdf:type schema:DefinedTerm
119 anzsrc-for:3142 schema:inDefinedTermSet anzsrc-for:
120 rdf:type schema:DefinedTerm
121 sg:pub.10.1007/s00412-014-0489-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004223733
122 https://doi.org/10.1007/s00412-014-0489-2
123 rdf:type schema:CreativeWork
124 sg:pub.10.1038/nature05432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017905987
125 https://doi.org/10.1038/nature05432
126 rdf:type schema:CreativeWork
127 sg:pub.10.1038/nature05465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042319022
128 https://doi.org/10.1038/nature05465
129 rdf:type schema:CreativeWork
130 sg:pub.10.1038/nature08647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022812644
131 https://doi.org/10.1038/nature08647
132 rdf:type schema:CreativeWork
133 sg:pub.10.1038/nature11920 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003048912
134 https://doi.org/10.1038/nature11920
135 rdf:type schema:CreativeWork
136 sg:pub.10.1038/nature14285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007234009
137 https://doi.org/10.1038/nature14285
138 rdf:type schema:CreativeWork
139 sg:pub.10.1038/nrc1548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027906138
140 https://doi.org/10.1038/nrc1548
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/nrc1569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002251969
143 https://doi.org/10.1038/nrc1569
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/nrc2820 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012705094
146 https://doi.org/10.1038/nrc2820
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/sj.bjc.6601234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011203305
149 https://doi.org/10.1038/sj.bjc.6601234
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1002/(sici)1097-4652(200003)182:3<311::aid-jcp1>3.0.co;2-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040757424
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1002/jcb.21698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039499151
154 rdf:type schema:CreativeWork
155 https://doi.org/10.1002/jso.10287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043593285
156 rdf:type schema:CreativeWork
157 https://doi.org/10.1002/path.1444 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013548862
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.ceb.2010.07.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004517774
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.cell.2009.10.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014449249
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.cell.2009.12.049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006869070
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/j.cell.2011.06.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044927218
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1016/j.cell.2011.07.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052894008
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/j.cub.2011.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022627276
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1016/j.molcel.2006.07.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022179585
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/j.molcel.2008.05.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042577158
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/j.molcel.2009.12.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013948145
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/j.molcel.2010.11.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003604838
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/s0140-6736(02)08739-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009511777
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/s0302-2838(02)00580-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045619954
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1034/j.1600-0714.2003.00116.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043798234
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1038/emboj.2011.404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030400958
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1038/sj.emboj.7601063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027987961
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1038/sj.emboj.7601075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003172621
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1046/j.0305-1846.2001.00333.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052756636
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1056/nejmoa033403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047425758
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1073/pnas.0604990103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043501647
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1073/pnas.0911500106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044523994
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1073/pnas.1509608112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029082981
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1073/pnas.82.10.3262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036404102
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1074/jbc.m109.039123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014503129
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1074/jbc.m111.218305 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017810177
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1074/jbc.m111.226332 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030636449
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1074/jbc.m113.532085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042604111
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1074/jbc.m114.608232 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027231397
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1074/jbc.m115.640383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047130932
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1074/jbc.m115.688424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058214050
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1074/jbc.m512921200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051278565
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1074/jbc.m608935200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026939157
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1080/02688690120114174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015995086
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1083/jcb.107.5.1623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002229533
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1083/jcb.201102003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005871957
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1093/emboj/20.8.2097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010219249
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1093/nar/gkq1222 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036541118
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1093/nar/gkr371 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012228174
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1101/gad.11.24.3365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028548738
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1101/gad.1759609 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049980699
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1101/gad.1883410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036815090
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1101/gad.1933010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035610853
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1101/gad.242404.114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041205054
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1101/gad.246827.114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012013105
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1101/gad.457807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049848236
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1111/j.1365-2990.2005.00618.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026965256
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1111/j.1463-1318.2004.00567.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010285696
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1111/j.1600-0560.1998.tb01747.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011730055
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/mmbr.68.1.109-131.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035970361
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1136/gut.46.6.746 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002836009
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1158/1078-0432.ccr-04-1776 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050314186
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1159/000078673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042013792
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1200/jco.2001.19.22.4259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074954341
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1200/jco.2003.04.121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064203544
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1593/neo.08216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037593382
266 rdf:type schema:CreativeWork
267 https://www.grid.ac/institutes/grid.255986.5 schema:Organization
 




Preview window. Press ESC to close (or click here)


...