Bioreactor for isolation of rare cells and methods of use


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Dennis E. Discher , Jae-Won Shin

ABSTRACT

The present invention relates to a bioreactor apparatus, and methods of use, for the isolation of rare blood cells, including hematopoietic stem cells and megakaryocytes. The apparatus includes a soft substrate and an anti-contractility agent, thereby providing a soft microenvironment to cultured cells. The apparatus of the invention is permissive for the survival of non-dividing cells while dividing cells are eliminated. This unique property allows for the simple isolation of rare blood cells without the use of costly equipment and antibodies. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Dennis E. Discher", 
        "type": "Person"
      }, 
      {
        "name": "Jae-Won Shin", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/pmic.200900758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001453601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.21698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002260090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.06.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002736945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003509222", 
          "https://doi.org/10.1038/nature02040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1197-1222", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005347530", 
          "https://doi.org/10.1038/nbt1197-1222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1017474108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007198340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1538-7836.2010.04009.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008160312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(92)90397-q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009939845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.202380099", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010145392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90443-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010910482"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.17.9021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013192496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-9525(98)01486-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015644059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jcis.2001.7633", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018524824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020039813", 
          "https://doi.org/10.1038/ncb734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022253901", 
          "https://doi.org/10.1038/nbt.1687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1191536", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022461982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.22891", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022765540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.8.12.2617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023653983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/002215549804600311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024225023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-7799(99)01398-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027867143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1081412", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028126150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9525(96)60040-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028136085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/mol.105.021832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028514333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2009.07.069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030330803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c400352200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035845985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.109.158261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035871212"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.22184", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037536108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/8756-3282(93)90202-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038731122"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2007-02-071589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045381339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045881928", 
          "https://doi.org/10.1038/nature08073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m501573200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046271264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jm980573a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055959108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la8033098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056163883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1163086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062458620"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "description": "

The present invention relates to a bioreactor apparatus, and methods of use, for the isolation of rare blood cells, including hematopoietic stem cells and megakaryocytes. The apparatus includes a soft substrate and an anti-contractility agent, thereby providing a soft microenvironment to cultured cells. The apparatus of the invention is permissive for the survival of non-dividing cells while dividing cells are eliminated. This unique property allows for the simple isolation of rare blood cells without the use of costly equipment and antibodies.

", "id": "sg:patent.US-9920295-B2", "keywords": [ "bioreactors", "isolation", "rare cell", "method", "invention", "blood cell", "hematopoietic stem cell", "megakaryocyte", "apparatus", "substrate", "contractility", "microenvironment", "cultured cell", "survival", "non-dividing cell", "cell", "unique property", "antibody" ], "name": "Bioreactor for isolation of rare cells and methods of use", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.29857.31", "type": "Organization" }, { "id": "https://www.grid.ac/institutes/grid.25879.31", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-9920295-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-03-07T15:30", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com.uberresearch.data.dev.patents-pipeline/full_run_10/sn-export/5eb3e5a348d7f117b22cc85fb0b02730/0000100128-0000348334/json_export_0782263a.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-9920295-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-9920295-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-9920295-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-9920295-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

152 TRIPLES      14 PREDICATES      66 URIs      25 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-9920295-B2 schema:about anzsrc-for:2581
2 schema:author Nec848728ff8f4b69bbc28e359c8fe876
3 schema:citation sg:pub.10.1038/nature02040
4 sg:pub.10.1038/nature08073
5 sg:pub.10.1038/nbt.1687
6 sg:pub.10.1038/nbt1197-1222
7 sg:pub.10.1038/ncb734
8 https://doi.org/10.1002/bit.21698
9 https://doi.org/10.1002/bit.22891
10 https://doi.org/10.1002/jcb.22184
11 https://doi.org/10.1002/pmic.200900758
12 https://doi.org/10.1006/jcis.2001.7633
13 https://doi.org/10.1016/0014-4827(92)90397-q
14 https://doi.org/10.1016/0092-8674(87)90443-0
15 https://doi.org/10.1016/0168-9525(96)60040-7
16 https://doi.org/10.1016/8756-3282(93)90202-l
17 https://doi.org/10.1016/j.cell.2006.06.044
18 https://doi.org/10.1016/j.cub.2009.07.069
19 https://doi.org/10.1016/s0167-7799(99)01398-0
20 https://doi.org/10.1016/s0168-9525(98)01486-3
21 https://doi.org/10.1021/jm980573a
22 https://doi.org/10.1021/la8033098
23 https://doi.org/10.1073/pnas.1017474108
24 https://doi.org/10.1073/pnas.202380099
25 https://doi.org/10.1073/pnas.93.17.9021
26 https://doi.org/10.1074/jbc.c400352200
27 https://doi.org/10.1074/jbc.m501573200
28 https://doi.org/10.1091/mbc.8.12.2617
29 https://doi.org/10.1111/j.1538-7836.2010.04009.x
30 https://doi.org/10.1124/jpet.109.158261
31 https://doi.org/10.1124/mol.105.021832
32 https://doi.org/10.1126/science.1081412
33 https://doi.org/10.1126/science.1163086
34 https://doi.org/10.1126/science.1191536
35 https://doi.org/10.1177/002215549804600311
36 https://doi.org/10.1182/blood-2007-02-071589
37 schema:description <p id="p-0001" num="0000">The present invention relates to a bioreactor apparatus, and methods of use, for the isolation of rare blood cells, including hematopoietic stem cells and megakaryocytes. The apparatus includes a soft substrate and an anti-contractility agent, thereby providing a soft microenvironment to cultured cells. The apparatus of the invention is permissive for the survival of non-dividing cells while dividing cells are eliminated. This unique property allows for the simple isolation of rare blood cells without the use of costly equipment and antibodies.</p>
38 schema:keywords antibody
39 apparatus
40 bioreactors
41 blood cell
42 cell
43 contractility
44 cultured cell
45 hematopoietic stem cell
46 invention
47 isolation
48 megakaryocyte
49 method
50 microenvironment
51 non-dividing cell
52 rare cell
53 substrate
54 survival
55 unique property
56 schema:name Bioreactor for isolation of rare cells and methods of use
57 schema:recipient https://www.grid.ac/institutes/grid.25879.31
58 https://www.grid.ac/institutes/grid.29857.31
59 schema:sameAs https://app.dimensions.ai/details/patent/US-9920295-B2
60 schema:sdDatePublished 2019-03-07T15:30
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher N58b23def83a64475bb541d5b2ca050e8
63 sgo:license sg:explorer/license/
64 sgo:sdDataset patents
65 rdf:type sgo:Patent
66 N0827d538c12842c6981b47f4391d27c1 rdf:first N7a4c749fdc5c4a87a911ff8c5ed607c9
67 rdf:rest rdf:nil
68 N58b23def83a64475bb541d5b2ca050e8 schema:name Springer Nature - SN SciGraph project
69 rdf:type schema:Organization
70 N7a4c749fdc5c4a87a911ff8c5ed607c9 schema:name Jae-Won Shin
71 rdf:type schema:Person
72 Nec848728ff8f4b69bbc28e359c8fe876 rdf:first Nfecdcbd38da741eca483462c1ee80955
73 rdf:rest N0827d538c12842c6981b47f4391d27c1
74 Nfecdcbd38da741eca483462c1ee80955 schema:name Dennis E. Discher
75 rdf:type schema:Person
76 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
77 rdf:type schema:DefinedTerm
78 sg:pub.10.1038/nature02040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003509222
79 https://doi.org/10.1038/nature02040
80 rdf:type schema:CreativeWork
81 sg:pub.10.1038/nature08073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045881928
82 https://doi.org/10.1038/nature08073
83 rdf:type schema:CreativeWork
84 sg:pub.10.1038/nbt.1687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022253901
85 https://doi.org/10.1038/nbt.1687
86 rdf:type schema:CreativeWork
87 sg:pub.10.1038/nbt1197-1222 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005347530
88 https://doi.org/10.1038/nbt1197-1222
89 rdf:type schema:CreativeWork
90 sg:pub.10.1038/ncb734 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020039813
91 https://doi.org/10.1038/ncb734
92 rdf:type schema:CreativeWork
93 https://doi.org/10.1002/bit.21698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002260090
94 rdf:type schema:CreativeWork
95 https://doi.org/10.1002/bit.22891 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022765540
96 rdf:type schema:CreativeWork
97 https://doi.org/10.1002/jcb.22184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037536108
98 rdf:type schema:CreativeWork
99 https://doi.org/10.1002/pmic.200900758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001453601
100 rdf:type schema:CreativeWork
101 https://doi.org/10.1006/jcis.2001.7633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018524824
102 rdf:type schema:CreativeWork
103 https://doi.org/10.1016/0014-4827(92)90397-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1009939845
104 rdf:type schema:CreativeWork
105 https://doi.org/10.1016/0092-8674(87)90443-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010910482
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1016/0168-9525(96)60040-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028136085
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/8756-3282(93)90202-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1038731122
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/j.cell.2006.06.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002736945
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1016/j.cub.2009.07.069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030330803
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/s0167-7799(99)01398-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027867143
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1016/s0168-9525(98)01486-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015644059
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1021/jm980573a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055959108
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1021/la8033098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056163883
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1073/pnas.1017474108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007198340
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1073/pnas.202380099 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010145392
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1073/pnas.93.17.9021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013192496
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1074/jbc.c400352200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035845985
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1074/jbc.m501573200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046271264
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1091/mbc.8.12.2617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023653983
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1111/j.1538-7836.2010.04009.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008160312
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1124/jpet.109.158261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035871212
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1124/mol.105.021832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028514333
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1126/science.1081412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028126150
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1126/science.1163086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062458620
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1126/science.1191536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022461982
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1177/002215549804600311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024225023
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1182/blood-2007-02-071589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045381339
150 rdf:type schema:CreativeWork
151 https://www.grid.ac/institutes/grid.25879.31 schema:Organization
152 https://www.grid.ac/institutes/grid.29857.31 schema:Organization
 




Preview window. Press ESC to close (or click here)


...