Nucleic acid amplification method


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Yoshiaki Yazawa , Takahide Yokoi , Chihiro Uematsu

ABSTRACT

A nucleic acid amplification method includes ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5). A 3′-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure. More... »

Related SciGraph Publications

  • 2010-01. Sequencing technologies — the next generation in NATURE REVIEWS GENETICS
  • 2008-10. Next-generation DNA sequencing in NATURE BIOTECHNOLOGY
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