3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Brian P. Stupi , Hong Li , Weidong Wu , Megan N. HERSH , David Hertzog , Sidney E. Morris , Michael L. Metzker

ABSTRACT

The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide. More... »

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