Methods and compositions for producing double allele knock outs


Ontology type: sgo:Patent     


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DATE

N/A

AUTHORS

Bo Yu , James Larrick

ABSTRACT

The present invention provides a method and compositions utilizing the CRISPR system to disrupt a target gene in eukaryotic cells to produce double allele knock outs. The method finds use in producing afucosylated antibodies with enhanced ADCC activity.

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