Clonal pre-amplification in emulsion


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Thomas Froehlich , Tobias Heckel

ABSTRACT

Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3′ ends and 5′ ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules. More... »

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