Method for exclusive selection of circularized DNA from monomolecular DNA in circularizing DNA molecules


Ontology type: sgo:Patent     


Patent Info

DATE

2016-08-16T00:00

AUTHORS

Shinichi Mizuno , Hidetoshi Ozawa , Koji Nagafuji , Takashi Okamura

ABSTRACT

The present invention provides a method for producing a circular DNA molecule having a specific structure that enables to distinguish circular DNA formed from a single DNA molecule (single-molecule circular DNA), from circular DNA formed from multiple DNA molecules (multiple-molecule circular DNA) and also from single-molecule circular DNA derived from the circular DNA formed from multiple DNA molecules. According to the present invention, only single-molecule circular DNA that is not derived from multiple-molecule circular DNA can be selected in the production of circular DNA. More... »

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