Compositions and methods directed to CRISPR/Cas genomic engineering systems


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Fangting Wu

ABSTRACT

The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the Streptococcus pyogenes (S. pyogenes) Cas9 protein, where the nucleotide sequence has been optimized for expression in mammalian cells. The specification also describes all-in-one systems for RNA-guided genome engineering in mammalian cells, including human cells. More... »

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