Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Carlos Lois-Caballe , David Baltimore , Xiao-Feng Qin , Irvin S. Y. Chen , Dong Sung An

ABSTRACT

In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells. More... »

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