3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Brian STUPI , Hong Li , Weidong Wu , Megan N. HERSH , David Hertzog , Sidney E. Morris , Michael L. Metzker

ABSTRACT

The present invention relates generally to 3′-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas: wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide. More... »

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