3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
Ontology type: sgo:Patent
Patent Info
DATE
N/A
AUTHORSBrian STUPI , Hong Li , Weidong Wu , Megan N. HERSH , David Hertzog , Sidney E. Morris , Michael L. Metzker
ABSTRACTThe present invention relates generally to 3′-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas: wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide. More... »
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"description": "The present invention relates generally to 3′-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas:
![\"embedded]()
![\"embedded]()
\nwherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.\n
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]Download the RDF metadata as: json-ld nt turtle xml License info
HOW TO GET THIS DATA PROGRAMMATICALLY:
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-8889860-B2'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-8889860-B2'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-8889860-B2'
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curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-8889860-B2'
This table displays all metadata directly associated to this object as RDF triples.
400 TRIPLES
14 PREDICATES
138 URIs
23 LITERALS
2 BLANK NODES