Enhanced gene expression in algae


Ontology type: sgo:Patent     


Patent Info

DATE

2014-08-26T00:00

AUTHORS

George Oyler , Julian Rosenberg

ABSTRACT

The invention provides eukaryotic unicellular algae engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression. More... »

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