Stable recombinant yeasts for fermenting xylose to ethanol


Ontology type: sgo:Patent     


Patent Info

DATE

2014-02-18T00:00

AUTHORS

Nancy W. Y. Ho , Zheng-Dao Chen

ABSTRACT

Described are recombinant yeast which ferment xylose to ethanol and which maintain their ability to do so when cultured for numerous generations in non-selective media. The preferred yeast contain multiple copies of integrated genes encoding xylose reductase, xylitol dehydrogenase, and xylulokinase fused to promoters which are non-glucose inhibited and which do not require xylose for induction. Also described are preferred methods for integrating multiple copies of exogenous DNA into host cells by transforming cells with replicative/integrative vectors, and then replicating the cells a number of times under selective pressure to promote retention of the vector in subsequent generations. The replicated vectors thus serve to integrate multiple copies of the exogenous DNA into the host cells throughout the replication/selection phase. Thereafter the selective pressure can be removed to promote loss of the vector in subsequent generations, leaving stable integrants of the exogenous DNA. More... »

Related SciGraph Publications

  • 1984-04. NADH-linked aldose reductase: the key to anaerobic alcoholic fermentation of xylose by yeasts in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1993-09. Cloning and improving the expression ofPichia stipitis xylose reductase gene inSaccharomyces cerevisiae in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1990-03. Xylulokinase activity in various yeasts includingSaccharomyces cerevisiae containing the cloned xylulokinase gene in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1989-04. The fermentation of xylose —an analysis of the expression of Bacillus and Actinoplanes xylose isomerase genes in yeast in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1990-06. Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1993-03. Xylose fermentation by Saccharomyces cerevisiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1997-08. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1990-12. Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant in CURRENT GENETICS
  • 1991-12. Enhanced Secretion of Human Nerve Growth Factor from Saccharomyces cerevisiae using an Advanced δ–Integration System in BIO/TECHNOLOGY
  • 1983-05. Fermentation of D-xylose to ethanol by a strain ofCandida shehatae in BIOTECHNOLOGY LETTERS
  • 1994-07. Multiple-copy integration in the yeast Yarrowia lipolytica in CURRENT GENETICS
  • 1992-11. Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C. albicans and in Saccharomyces cerevisiae in MOLECULAR GENETICS AND GENOMICS
  • 1991-02. Optimisation of a host/vector system for heterologous gene expression by Hansenula polymorpha in CURRENT GENETICS
  • 1984-05. Cloning and characterization of the xyl genes from Escherichia coli in MOLECULAR GENETICS AND GENOMICS
  • 1991-11. Xylitol Production by Recombinant Saccharomyces Cerevisiae in BIO/TECHNOLOGY
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