Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells


Ontology type: sgo:Patent     


Patent Info

DATE

2013-12-31T00:00

AUTHORS

Shouguang Jin , CANDACE BICHSEL

ABSTRACT

A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2995", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Shouguang Jin", 
        "type": "Person"
      }, 
      {
        "name": "CANDACE BICHSEL", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1091/mbc.e05-06-0572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000015217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1152092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000037346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.5411905.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000379095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.5411905.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000379095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.5411905.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000379095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05934", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002347899", 
          "https://doi.org/10.1038/nature05934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002503252", 
          "https://doi.org/10.1038/nature08436"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1374", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002744128", 
          "https://doi.org/10.1038/nbt1374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jconrel.2006.10.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005812548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cfg.405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008253226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0605949103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009808315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/17460751.2.5.795", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010901033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010904856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010904856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011057005", 
          "https://doi.org/10.1038/nbt780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2005-0292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011281633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2005-0292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011281633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017686107", 
          "https://doi.org/10.1038/nature07061"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.23.13899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020669591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.r600026200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021064029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1162494", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021871278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.01.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022080492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.01.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022080492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031832630", 
          "https://doi.org/10.1038/nrmicro1526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031832630", 
          "https://doi.org/10.1038/nrmicro1526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033056939", 
          "https://doi.org/10.1038/nrmicro2199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033056939", 
          "https://doi.org/10.1038/nrmicro2199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1303605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033143053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.6251991.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035575919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.6251991.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035575919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.1997.6251991.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035575919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1151526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048905674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051466228", 
          "https://doi.org/10.1038/nbt.1502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1164270", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062458687"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-12-31T00:00", 
    "description": "

A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.

", "id": "sg:patent.US-8617888-B2", "keywords": [ "delivery", "nuclear protein", "differentiated cell", "P. aeruginosa", "type III secretion system", "selected protein", "host cell", "Cre recombinase", "embryonic stem", "Pluripotent Stem", "cell", "method", "directed differentiation", "insertional mutagenesis", "route", "stem cell renewal", "cell-based therapy" ], "name": "Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.15276.37", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-8617888-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:12", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00711.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-8617888-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-8617888-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-8617888-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-8617888-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

132 TRIPLES      15 PREDICATES      58 URIs      25 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-8617888-B2 schema:about anzsrc-for:2581
2 anzsrc-for:2995
3 schema:author N851233ac1d4043f289898c30d6482f9d
4 schema:citation sg:pub.10.1038/nature05934
5 sg:pub.10.1038/nature07061
6 sg:pub.10.1038/nature08436
7 sg:pub.10.1038/nbt.1502
8 sg:pub.10.1038/nbt1374
9 sg:pub.10.1038/nbt780
10 sg:pub.10.1038/nrmicro1526
11 sg:pub.10.1038/nrmicro2199
12 https://doi.org/10.1002/cfg.405
13 https://doi.org/10.1016/j.cell.2006.07.024
14 https://doi.org/10.1016/j.cell.2007.11.019
15 https://doi.org/10.1016/j.cell.2009.01.023
16 https://doi.org/10.1016/j.jconrel.2006.10.031
17 https://doi.org/10.1046/j.1365-2958.1997.5411905.x
18 https://doi.org/10.1046/j.1365-2958.1997.6251991.x
19 https://doi.org/10.1073/pnas.0605949103
20 https://doi.org/10.1073/pnas.95.23.13899
21 https://doi.org/10.1074/jbc.r600026200
22 https://doi.org/10.1091/mbc.e05-06-0572
23 https://doi.org/10.1101/gad.1303605
24 https://doi.org/10.1126/science.1151526
25 https://doi.org/10.1126/science.1152092
26 https://doi.org/10.1126/science.1162494
27 https://doi.org/10.1126/science.1164270
28 https://doi.org/10.1634/stemcells.2005-0292
29 https://doi.org/10.2217/17460751.2.5.795
30 schema:datePublished 2013-12-31T00:00
31 schema:description <p num="p-0001">A modified <i>P. aeruginosa </i>type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.</p>
32 schema:keywords Cre recombinase
33 P. aeruginosa
34 Pluripotent Stem
35 cell
36 cell-based therapy
37 delivery
38 differentiated cell
39 directed differentiation
40 embryonic stem
41 host cell
42 insertional mutagenesis
43 method
44 nuclear protein
45 route
46 selected protein
47 stem cell renewal
48 type III secretion system
49 schema:name Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
50 schema:recipient https://www.grid.ac/institutes/grid.15276.37
51 schema:sameAs https://app.dimensions.ai/details/patent/US-8617888-B2
52 schema:sdDatePublished 2019-04-18T10:12
53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
54 schema:sdPublisher N24469fb5f069466c9dd0a1c74be27ef4
55 sgo:license sg:explorer/license/
56 sgo:sdDataset patents
57 rdf:type sgo:Patent
58 N24469fb5f069466c9dd0a1c74be27ef4 schema:name Springer Nature - SN SciGraph project
59 rdf:type schema:Organization
60 N7a5c89e7d2ea4a318a0e1f0b34a6b171 rdf:first Nb23723e466754052a47a9b0bf7689194
61 rdf:rest rdf:nil
62 N851233ac1d4043f289898c30d6482f9d rdf:first Nd2779837594c4be8b752fc28f1821b44
63 rdf:rest N7a5c89e7d2ea4a318a0e1f0b34a6b171
64 Nb23723e466754052a47a9b0bf7689194 schema:name CANDACE BICHSEL
65 rdf:type schema:Person
66 Nd2779837594c4be8b752fc28f1821b44 schema:name Shouguang Jin
67 rdf:type schema:Person
68 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
69 rdf:type schema:DefinedTerm
70 anzsrc-for:2995 schema:inDefinedTermSet anzsrc-for:
71 rdf:type schema:DefinedTerm
72 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
73 https://doi.org/10.1038/nature05934
74 rdf:type schema:CreativeWork
75 sg:pub.10.1038/nature07061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017686107
76 https://doi.org/10.1038/nature07061
77 rdf:type schema:CreativeWork
78 sg:pub.10.1038/nature08436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002503252
79 https://doi.org/10.1038/nature08436
80 rdf:type schema:CreativeWork
81 sg:pub.10.1038/nbt.1502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466228
82 https://doi.org/10.1038/nbt.1502
83 rdf:type schema:CreativeWork
84 sg:pub.10.1038/nbt1374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002744128
85 https://doi.org/10.1038/nbt1374
86 rdf:type schema:CreativeWork
87 sg:pub.10.1038/nbt780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011057005
88 https://doi.org/10.1038/nbt780
89 rdf:type schema:CreativeWork
90 sg:pub.10.1038/nrmicro1526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031832630
91 https://doi.org/10.1038/nrmicro1526
92 rdf:type schema:CreativeWork
93 sg:pub.10.1038/nrmicro2199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033056939
94 https://doi.org/10.1038/nrmicro2199
95 rdf:type schema:CreativeWork
96 https://doi.org/10.1002/cfg.405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008253226
97 rdf:type schema:CreativeWork
98 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
99 rdf:type schema:CreativeWork
100 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
101 rdf:type schema:CreativeWork
102 https://doi.org/10.1016/j.cell.2009.01.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022080492
103 rdf:type schema:CreativeWork
104 https://doi.org/10.1016/j.jconrel.2006.10.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005812548
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1046/j.1365-2958.1997.5411905.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000379095
107 rdf:type schema:CreativeWork
108 https://doi.org/10.1046/j.1365-2958.1997.6251991.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035575919
109 rdf:type schema:CreativeWork
110 https://doi.org/10.1073/pnas.0605949103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009808315
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1073/pnas.95.23.13899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020669591
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1074/jbc.r600026200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021064029
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1091/mbc.e05-06-0572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000015217
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1101/gad.1303605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033143053
119 rdf:type schema:CreativeWork
120 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
121 rdf:type schema:CreativeWork
122 https://doi.org/10.1126/science.1152092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000037346
123 rdf:type schema:CreativeWork
124 https://doi.org/10.1126/science.1162494 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021871278
125 rdf:type schema:CreativeWork
126 https://doi.org/10.1126/science.1164270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062458687
127 rdf:type schema:CreativeWork
128 https://doi.org/10.1634/stemcells.2005-0292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011281633
129 rdf:type schema:CreativeWork
130 https://doi.org/10.2217/17460751.2.5.795 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010901033
131 rdf:type schema:CreativeWork
132 https://www.grid.ac/institutes/grid.15276.37 schema:Organization
 




Preview window. Press ESC to close (or click here)


...