Swellable (meth)acrylate surfaces for culturing cells in chemically defined media


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Andrei G. Fadeev , Jennifer Gehman , Arthur Winston Martin , Zara Melkoumian , Christopher B. Shogbon , David Michael Weber , Yue Zhou

ABSTRACT

Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation. More... »

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