Polymerase inhibitor and method of using same


Ontology type: sgo:Patent     


Patent Info

DATE

2012-11-20T00:00

AUTHORS

Michael James Moser , David J. Marshall , James R. Prudent , Cristopher V. Van Hout , Christine A. Larsen

ABSTRACT

The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension. More... »

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