Cosmid vector for plant transformation and use thereof


Ontology type: sgo:Patent     


Patent Info

DATE

2012-10-30T00:00

AUTHORS

Yoshimitsu Takakura , Toshihiko Komari , Yuji Ishida , Toshiyuki Komori , Yukoh Hiei , Toshiki Mine , Teruyuki Imayama

ABSTRACT

The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups;2) they contain the trfA1 gene of an IncP plasmid;3) they contain an oriT of an IncP plasmid;4) they contain the incC1 gene of an IncP plasmid;5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA;6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene. More... »

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The present invention aims to provide novel vectors for plant transformation.

The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that:\n

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    • 2) they contain the trfA1 gene of an IncP plasmid;
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    • 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium;
    • 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium;
    • 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and
    • 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.
\n

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