Trace mRNA amplification method and use thereof


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Hiroshi Nojima , Takahiro Tougan , Daisuke Okuzaki

ABSTRACT

A method for amplifying a trace amount of mRNA includes adding a dummy RNA to a solution containing the trace amount of mRNA, so as to prepare a mixed solution; synthesizing an anti-sense DNA by reverse transcription, which uses the mixed solution as a template; synthesizing a sense DNA which is complementary to the anti-sense DNA thus synthesized, so as to generate a double-strand DNA made of the sense DNA and the anti-sense DNA; ligating an RNA polymerase promoter sequence to the double-strand DNA thus generated, on a sense DNA 5′ end side of the double-strand DNA, so as to prepare a double-strand DNA for amplification; and amplifying, by using RNA polymerase, an RNA from the double-strand DNA for amplification. More... »

Related SciGraph Publications

  • 2000-04. High-fidelity mRNA amplification for gene profiling in NATURE BIOTECHNOLOGY
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