Massive parallel method for decoding DNA and RNA


Ontology type: sgo:Patent     


Patent Info

DATE

2012-01-03T00:00

AUTHORS

Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki

ABSTRACT

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose. More... »

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