Methods for expression and purification of pegylated recombinant human growth hormone containing a non-naturally encoded keto amino acid


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Ying Buechler , Ricky Lieu , Michael Ong , Stuart Bussell , Nick Knudsen , Ho Sung Cho

ABSTRACT

The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules. More... »

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    The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.

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