Solid support assay systems and methods utilizing non-standard bases


Ontology type: sgo:Patent     


Patent Info

DATE

2011-02-22T00:00

AUTHORS

James Prudent , David Marshall , Christopher Sherrill , Eric Roesch , Craig Richmond , Christopher Scherrer , Jennifer Grenier , Jerod Ptacin

ABSTRACT

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay. More... »

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