Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol


Ontology type: sgo:Patent     


Patent Info

DATE

2010-09-28T00:00

AUTHORS

Mark J. Burk , Christophe H. Schilling , Anthony P. Burgard , John D. Trawick

ABSTRACT

The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA. More... »

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        "description": "

    The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

    ", "id": "sg:patent.US-7803589-B2", "keywords": [ "method", "organism", "synthesis", "carbon source", "methanol", "invention", "microbial organism", "pathway", "capability", "syngas", "embodiment", "microorganism", "exogenous protein", "CO", "acetyl coenzyme", "tetrahydrofolate", "desired product", "wherein", "absence", "nucleic acid", "enzyme", "protein", "culturing", "pathway enzyme", "sufficient amount", "condition", "period" ], "name": "Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.420355.5", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-7803589-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:06", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00113.jsonl", "type": "Patent" } ]
     

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    868 schema:datePublished 2010-09-28T00:00
    869 schema:description <p num="p-0001">The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO<sub>2 </sub>and/or H<sub>2 </sub>to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO<sub>2 </sub>and H<sub>2 </sub>to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO<sub>2 </sub>and/or H<sub>2</sub>, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.</p>
    870 schema:keywords CO
    871 absence
    872 acetyl coenzyme
    873 capability
    874 carbon source
    875 condition
    876 culturing
    877 desired product
    878 embodiment
    879 enzyme
    880 exogenous protein
    881 invention
    882 methanol
    883 method
    884 microbial organism
    885 microorganism
    886 nucleic acid
    887 organism
    888 pathway
    889 pathway enzyme
    890 period
    891 protein
    892 sufficient amount
    893 syngas
    894 synthesis
    895 tetrahydrofolate
    896 wherein
    897 schema:name Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
    898