Methylated CpG island amplification (MCA)


Ontology type: sgo:Patent     


Patent Info

DATE

2010-04-20T00:00

AUTHORS

Jean-Pierre Issa , Stephen Baylin , Minoru Toyota

ABSTRACT

The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluation the response of a cell to an agent A kit useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Jean-Pierre Issa", 
        "type": "Person"
      }, 
      {
        "name": "Stephen Baylin", 
        "type": "Person"
      }, 
      {
        "name": "Minoru Toyota", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1006/scbi.1999.0135", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003503023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02736773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029799669", 
          "https://doi.org/10.1007/bf02736773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02736773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029799669", 
          "https://doi.org/10.1007/bf02736773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.6.2284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035787918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.2910620303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036375440"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004390050689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046179168", 
          "https://doi.org/10.1007/s004390050689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004390050689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046179168", 
          "https://doi.org/10.1007/s004390050689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8438152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb05755.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082784709"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-04-20T00:00", 
    "description": "

The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluation the response of a cell to an agent A kit useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones.

", "id": "sg:patent.US-7700324-B1", "keywords": [ "CpG island", "invention", "method", "CpG", "nucleic acid", "endonuclease", "CpG site", "sample", "amplification", "specific oligonucleotides", "associated disorder", "Evaluation Study as Topic", "cell", "agent", "kit", "nucleic acid sequence" ], "name": "Methylated CpG island amplification (MCA)", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.21107.35", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-7700324-B1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:05", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00051.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-7700324-B1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-7700324-B1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-7700324-B1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-7700324-B1'


 

This table displays all metadata directly associated to this object as RDF triples.

69 TRIPLES      15 PREDICATES      37 URIs      24 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-7700324-B1 schema:about anzsrc-for:2620
2 schema:author Nb211508b70c34194b0ec35dc8cc9029a
3 schema:citation sg:pub.10.1007/bf02736773
4 sg:pub.10.1007/s004390050689
5 https://doi.org/10.1002/ijc.2910620303
6 https://doi.org/10.1002/j.1460-2075.1993.tb05755.x
7 https://doi.org/10.1006/scbi.1999.0135
8 https://doi.org/10.1073/pnas.94.6.2284
9 https://doi.org/10.1126/science.8438152
10 schema:datePublished 2010-04-20T00:00
11 schema:description <p num="p-0001">The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluation the response of a cell to an agent A kit useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones.</p>
12 schema:keywords CpG
13 CpG island
14 CpG site
15 Evaluation Study as Topic
16 agent
17 amplification
18 associated disorder
19 cell
20 endonuclease
21 invention
22 kit
23 method
24 nucleic acid
25 nucleic acid sequence
26 sample
27 specific oligonucleotides
28 schema:name Methylated CpG island amplification (MCA)
29 schema:recipient https://www.grid.ac/institutes/grid.21107.35
30 schema:sameAs https://app.dimensions.ai/details/patent/US-7700324-B1
31 schema:sdDatePublished 2019-04-18T10:05
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher Nb30b84dd6f914e3cb450bfc57a20e835
34 sgo:license sg:explorer/license/
35 sgo:sdDataset patents
36 rdf:type sgo:Patent
37 N14274ecd5c9f4a6188be343649c28a6b rdf:first N26c4f3335e9048159c74b09cf3c3c55f
38 rdf:rest rdf:nil
39 N26c4f3335e9048159c74b09cf3c3c55f schema:name Minoru Toyota
40 rdf:type schema:Person
41 N325832ad9dbc4f8c83a90d18aa03125d rdf:first Nd5c429f0600f41bd93160d8dafe75213
42 rdf:rest N14274ecd5c9f4a6188be343649c28a6b
43 Nb211508b70c34194b0ec35dc8cc9029a rdf:first Ncf794333f1824e7ea9eabbca57fa2634
44 rdf:rest N325832ad9dbc4f8c83a90d18aa03125d
45 Nb30b84dd6f914e3cb450bfc57a20e835 schema:name Springer Nature - SN SciGraph project
46 rdf:type schema:Organization
47 Ncf794333f1824e7ea9eabbca57fa2634 schema:name Jean-Pierre Issa
48 rdf:type schema:Person
49 Nd5c429f0600f41bd93160d8dafe75213 schema:name Stephen Baylin
50 rdf:type schema:Person
51 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
52 rdf:type schema:DefinedTerm
53 sg:pub.10.1007/bf02736773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029799669
54 https://doi.org/10.1007/bf02736773
55 rdf:type schema:CreativeWork
56 sg:pub.10.1007/s004390050689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046179168
57 https://doi.org/10.1007/s004390050689
58 rdf:type schema:CreativeWork
59 https://doi.org/10.1002/ijc.2910620303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036375440
60 rdf:type schema:CreativeWork
61 https://doi.org/10.1002/j.1460-2075.1993.tb05755.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082784709
62 rdf:type schema:CreativeWork
63 https://doi.org/10.1006/scbi.1999.0135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003503023
64 rdf:type schema:CreativeWork
65 https://doi.org/10.1073/pnas.94.6.2284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035787918
66 rdf:type schema:CreativeWork
67 https://doi.org/10.1126/science.8438152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655586
68 rdf:type schema:CreativeWork
69 https://www.grid.ac/institutes/grid.21107.35 schema:Organization
 




Preview window. Press ESC to close (or click here)


...