Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Paul D. Taylor , Reyes Candau , Gary F. Gerard

ABSTRACT

The invention relates to methods for identifying the sequence of one or more variant nucleotides in nucleic acid molecules. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3′ side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a Double-Stranded Linker with a degenerate 3′-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out. More... »

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