Nucleic acid amplification using non-standard bases


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

James R. Prudent , Scott C. Johnson , Michael J. Moser , David J. Marshall

ABSTRACT

The present invention provides methods, kits and compositions for high fidelity nucleic acid amplification reactions of target nucleic acids such that specific amplification products that incorporate non-standard bases are produced. The amplification reactions can either be linear or exponential. In some embodiments, the fidelity of the amplification reaction is high enough such that a majority of the amplification products maintain non-standard bases at specific sites designated by the user after a specified number of amplification cycles. In some embodiments, the ratio of non-standard nucleoside triphosphates is greater than the amount of standard nucleoside triphosphates initially present in the reaction mixture. More... »

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