Primer extension methods for production of high specific activity nucleic acid probes


Ontology type: sgo:Patent     


Patent Info

DATE

2006-11-14T00:00

AUTHORS

Mark Aaron Behlke , Eric Jeffrey Devor , Shale Anthony James , Joseph Alan Walder

ABSTRACT

The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Mark Aaron Behlke", 
        "type": "Person"
      }, 
      {
        "name": "Eric Jeffrey Devor", 
        "type": "Person"
      }, 
      {
        "name": "Shale Anthony James", 
        "type": "Person"
      }, 
      {
        "name": "Joseph Alan Walder", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1600-065x.1995.tb00099.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004455284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/309418a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009921132", 
          "https://doi.org/10.1038/309418a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/25.22.4447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013099153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.179.1.323", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016985351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/343033a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023013667", 
          "https://doi.org/10.1038/343033a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.11.6633", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044308824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s1355838299991033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054924064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-11-14T00:00", 
    "description": "

The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.

", "id": "sg:patent.US-7135284-B1", "keywords": [ "primer extension", "high specific activity", "invention", "labeling technique", "nucleic acid probe", "specific activity", "labeling method", "method", "labeled nucleotide", "RNA", "DNA", "probe", "reaction mixture", "kit", "practice", "composition" ], "name": "Primer extension methods for production of high specific activity nucleic acid probes", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.420360.3", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-7135284-B1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:29", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03179.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-7135284-B1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-7135284-B1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-7135284-B1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-7135284-B1'


 

This table displays all metadata directly associated to this object as RDF triples.

76 TRIPLES      15 PREDICATES      38 URIs      24 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-7135284-B1 schema:about anzsrc-for:2620
2 schema:author N52b50db7f4994421a58fd9117f609573
3 schema:citation sg:pub.10.1038/309418a0
4 sg:pub.10.1038/343033a0
5 https://doi.org/10.1017/s1355838299991033
6 https://doi.org/10.1073/pnas.78.11.6633
7 https://doi.org/10.1084/jem.179.1.323
8 https://doi.org/10.1093/nar/25.22.4447
9 https://doi.org/10.1097/00007691-199802000-00001
10 https://doi.org/10.1111/j.1600-065x.1995.tb00099.x
11 schema:datePublished 2006-11-14T00:00
12 schema:description <p num="p-0001">The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.</p>
13 schema:keywords DNA
14 RNA
15 composition
16 high specific activity
17 invention
18 kit
19 labeled nucleotide
20 labeling method
21 labeling technique
22 method
23 nucleic acid probe
24 practice
25 primer extension
26 probe
27 reaction mixture
28 specific activity
29 schema:name Primer extension methods for production of high specific activity nucleic acid probes
30 schema:recipient https://www.grid.ac/institutes/grid.420360.3
31 schema:sameAs https://app.dimensions.ai/details/patent/US-7135284-B1
32 schema:sdDatePublished 2019-04-18T10:29
33 schema:sdLicense https://scigraph.springernature.com/explorer/license/
34 schema:sdPublisher Nfb7811fa1e4d4f6fbcc8852b95e5f648
35 sgo:license sg:explorer/license/
36 sgo:sdDataset patents
37 rdf:type sgo:Patent
38 N131cc12f533c4fc9950e626f5b9c2d91 schema:name Mark Aaron Behlke
39 rdf:type schema:Person
40 N365f6eecb68c4ca6854ba9f471f00616 rdf:first N56fe8ce82c1a48e2849d6ac6c5eb5bc5
41 rdf:rest Nd13d73ebf8f44b749b6c8367c8fd2cb0
42 N52b50db7f4994421a58fd9117f609573 rdf:first N131cc12f533c4fc9950e626f5b9c2d91
43 rdf:rest N725e55df81c04c2e99146ad49fef5a4e
44 N56fe8ce82c1a48e2849d6ac6c5eb5bc5 schema:name Shale Anthony James
45 rdf:type schema:Person
46 N725e55df81c04c2e99146ad49fef5a4e rdf:first N896ff61fe929436e9d9eb6adc69a4277
47 rdf:rest N365f6eecb68c4ca6854ba9f471f00616
48 N896ff61fe929436e9d9eb6adc69a4277 schema:name Eric Jeffrey Devor
49 rdf:type schema:Person
50 Nb8bb95a3bcc64737b45d58d4003ddfa0 schema:name Joseph Alan Walder
51 rdf:type schema:Person
52 Nd13d73ebf8f44b749b6c8367c8fd2cb0 rdf:first Nb8bb95a3bcc64737b45d58d4003ddfa0
53 rdf:rest rdf:nil
54 Nfb7811fa1e4d4f6fbcc8852b95e5f648 schema:name Springer Nature - SN SciGraph project
55 rdf:type schema:Organization
56 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
57 rdf:type schema:DefinedTerm
58 sg:pub.10.1038/309418a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009921132
59 https://doi.org/10.1038/309418a0
60 rdf:type schema:CreativeWork
61 sg:pub.10.1038/343033a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023013667
62 https://doi.org/10.1038/343033a0
63 rdf:type schema:CreativeWork
64 https://doi.org/10.1017/s1355838299991033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054924064
65 rdf:type schema:CreativeWork
66 https://doi.org/10.1073/pnas.78.11.6633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044308824
67 rdf:type schema:CreativeWork
68 https://doi.org/10.1084/jem.179.1.323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016985351
69 rdf:type schema:CreativeWork
70 https://doi.org/10.1093/nar/25.22.4447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013099153
71 rdf:type schema:CreativeWork
72 https://doi.org/10.1097/00007691-199802000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060274499
73 rdf:type schema:CreativeWork
74 https://doi.org/10.1111/j.1600-065x.1995.tb00099.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004455284
75 rdf:type schema:CreativeWork
76 https://www.grid.ac/institutes/grid.420360.3 schema:Organization
 




Preview window. Press ESC to close (or click here)


...