Primer extension methods for production of high specific activity nucleic acid probes


Ontology type: sgo:Patent     


Patent Info

DATE

2006-11-14T00:00

AUTHORS

Mark Aaron Behlke , Eric Jeffrey Devor , Shale Anthony James , Joseph Alan Walder

ABSTRACT

The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Mark Aaron Behlke", 
        "type": "Person"
      }, 
      {
        "name": "Eric Jeffrey Devor", 
        "type": "Person"
      }, 
      {
        "name": "Shale Anthony James", 
        "type": "Person"
      }, 
      {
        "name": "Joseph Alan Walder", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1600-065x.1995.tb00099.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004455284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/309418a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009921132", 
          "https://doi.org/10.1038/309418a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/25.22.4447", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013099153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.179.1.323", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016985351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/343033a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023013667", 
          "https://doi.org/10.1038/343033a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.11.6633", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044308824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s1355838299991033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054924064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007691-199802000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060274499"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-11-14T00:00", 
    "description": "

The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.

", "id": "sg:patent.US-7135284-B1", "keywords": [ "primer extension", "high specific activity", "invention", "labeling technique", "nucleic acid probe", "specific activity", "labeling method", "method", "labeled nucleotide", "RNA", "DNA", "probe", "reaction mixture", "kit", "practice", "composition" ], "name": "Primer extension methods for production of high specific activity nucleic acid probes", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.420360.3", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-7135284-B1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:29", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03179.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-7135284-B1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-7135284-B1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-7135284-B1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-7135284-B1'


 

This table displays all metadata directly associated to this object as RDF triples.

76 TRIPLES      15 PREDICATES      38 URIs      24 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-7135284-B1 schema:about anzsrc-for:2620
2 schema:author Na1d25e4dc6d240119548bb3d7177a9d7
3 schema:citation sg:pub.10.1038/309418a0
4 sg:pub.10.1038/343033a0
5 https://doi.org/10.1017/s1355838299991033
6 https://doi.org/10.1073/pnas.78.11.6633
7 https://doi.org/10.1084/jem.179.1.323
8 https://doi.org/10.1093/nar/25.22.4447
9 https://doi.org/10.1097/00007691-199802000-00001
10 https://doi.org/10.1111/j.1600-065x.1995.tb00099.x
11 schema:datePublished 2006-11-14T00:00
12 schema:description <p num="p-0001">The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.</p>
13 schema:keywords DNA
14 RNA
15 composition
16 high specific activity
17 invention
18 kit
19 labeled nucleotide
20 labeling method
21 labeling technique
22 method
23 nucleic acid probe
24 practice
25 primer extension
26 probe
27 reaction mixture
28 specific activity
29 schema:name Primer extension methods for production of high specific activity nucleic acid probes
30 schema:recipient https://www.grid.ac/institutes/grid.420360.3
31 schema:sameAs https://app.dimensions.ai/details/patent/US-7135284-B1
32 schema:sdDatePublished 2019-04-18T10:29
33 schema:sdLicense https://scigraph.springernature.com/explorer/license/
34 schema:sdPublisher N60023df14b96423eb2c72333698c0f0f
35 sgo:license sg:explorer/license/
36 sgo:sdDataset patents
37 rdf:type sgo:Patent
38 N3ac5f8dff73f42a5abcc5e7162f12b58 schema:name Mark Aaron Behlke
39 rdf:type schema:Person
40 N56e4cfc7c9e04850a1be0650153d5c3b schema:name Joseph Alan Walder
41 rdf:type schema:Person
42 N60023df14b96423eb2c72333698c0f0f schema:name Springer Nature - SN SciGraph project
43 rdf:type schema:Organization
44 N7d69178bb3ae428fb885051b9054964e rdf:first N56e4cfc7c9e04850a1be0650153d5c3b
45 rdf:rest rdf:nil
46 N912fde6bcab64f4ea00d6edfd35695d3 rdf:first Nd0f24261b9a841caac0c6e20f3fa6ddd
47 rdf:rest N9889a92b36684ecebdd03bccae30f78c
48 N9889a92b36684ecebdd03bccae30f78c rdf:first Ne44de3bd0e994f499f438b8a2411d570
49 rdf:rest N7d69178bb3ae428fb885051b9054964e
50 Na1d25e4dc6d240119548bb3d7177a9d7 rdf:first N3ac5f8dff73f42a5abcc5e7162f12b58
51 rdf:rest N912fde6bcab64f4ea00d6edfd35695d3
52 Nd0f24261b9a841caac0c6e20f3fa6ddd schema:name Eric Jeffrey Devor
53 rdf:type schema:Person
54 Ne44de3bd0e994f499f438b8a2411d570 schema:name Shale Anthony James
55 rdf:type schema:Person
56 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
57 rdf:type schema:DefinedTerm
58 sg:pub.10.1038/309418a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009921132
59 https://doi.org/10.1038/309418a0
60 rdf:type schema:CreativeWork
61 sg:pub.10.1038/343033a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023013667
62 https://doi.org/10.1038/343033a0
63 rdf:type schema:CreativeWork
64 https://doi.org/10.1017/s1355838299991033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054924064
65 rdf:type schema:CreativeWork
66 https://doi.org/10.1073/pnas.78.11.6633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044308824
67 rdf:type schema:CreativeWork
68 https://doi.org/10.1084/jem.179.1.323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016985351
69 rdf:type schema:CreativeWork
70 https://doi.org/10.1093/nar/25.22.4447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013099153
71 rdf:type schema:CreativeWork
72 https://doi.org/10.1097/00007691-199802000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060274499
73 rdf:type schema:CreativeWork
74 https://doi.org/10.1111/j.1600-065x.1995.tb00099.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004455284
75 rdf:type schema:CreativeWork
76 https://www.grid.ac/institutes/grid.420360.3 schema:Organization
 




Preview window. Press ESC to close (or click here)


...