Methods for producing and transforming cassava protoplasts


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Richard G. F. Visser , Christiaan J. J. Raemakers , Evert Jacobson , Johanna Elizabeth M. Bergorvoet van Deelen

ABSTRACT

The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby.Starch isolated from tubers of such cassava plants have an increased amylopectin content. The starch and a method for isolating it from said plants also forms part of the invention. More... »

Related SciGraph Publications

  • 1988-08. Sugarcane protoplasts: factors affecting division and plant regeneration in PLANT CELL REPORTS
  • 1972-06. Development and differentiation of haploid Lycopersicon esculentum (tomato) in PLANTA
  • 1979-05. Somatic hybrids between unilateral cross-incompatible Petunia species in THEORETICAL AND APPLIED GENETICS
  • 1992-02. Agrobacterium tumefaciens-mediated transformation of recalcitrant crops in PLANT MOLECULAR BIOLOGY REPORTER
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  • 1995-01. Advances in alternative DNA delivery techniques in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1985-05. Direct gene transfer to cells of a graminaceous monocot in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Gene transfer to cereal cells mediated by protoplast transformation in MOLECULAR GENETICS AND GENOMICS
  • 1988-12. Transgenic plants of Orchardgrass (Dactylis glomerata L.) from protoplasts in PLANT CELL REPORTS
  • 1987-06. In vitro somatic embryogenesis and plant regeneration of cassava in PLANT CELL REPORTS
  • 1991-12. Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum in THEORETICAL AND APPLIED GENETICS
  • 1989-03. Fertile transgenic rice plants regenerated from transformed protoplasts in NATURE
  • 1987-07. Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene in PLANT CELL REPORTS
  • 1988-12. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response in THEORETICAL AND APPLIED GENETICS
  • 1988-06. Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato in POTATO RESEARCH
  • 1993-12. Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato in PLANT MOLECULAR BIOLOGY
  • 1988-09. Transgenic Rice Plants After Direct Gene Transfer into Protoplasts in NATURE BIOTECHNOLOGY
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    The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby.

    Starch isolated from tubers of such cassava plants have an increased amylopectin content. The starch and a method for isolating it from said plants also forms part of the invention.

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