Nanoparticles having oligonucleotides attached thereto and uses therefor


Ontology type: sgo:Patent     


Patent Info

DATE

2004-01-06T00:00

AUTHORS

Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton

ABSTRACT

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/3021", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Chad A. Mirkin", 
        "type": "Person"
      }, 
      {
        "name": "Robert L. Letsinger", 
        "type": "Person"
      }, 
      {
        "name": "Robert C. Mucic", 
        "type": "Person"
      }, 
      {
        "name": "James J. Storhoff", 
        "type": "Person"
      }, 
      {
        "name": "Robert Elghanian", 
        "type": "Person"
      }, 
      {
        "name": "Thomas A. Taton", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/nar/30.7.1558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000482521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la950776r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000739633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la950776r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000739633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bioeng.3.1.335", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002076643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.277.5329.1078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010525136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bb.23.060194.002545", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011235010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382609a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013811778", 
          "https://doi.org/10.1038/382609a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(77)90182-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015148835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/adma.19950070907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017153621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/adma.19950070907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017153621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/382607a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030717946", 
          "https://doi.org/10.1038/382607a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(84)90518-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034491884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/350631a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034775610", 
          "https://doi.org/10.1038/350631a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja010437m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041739689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja010437m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041739689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/cc9960000555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042820289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0040-4039(96)01381-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047699773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1296-441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051306917", 
          "https://doi.org/10.1038/ng1296-441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.4.696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051767673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac00100a008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054971987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc990152n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055156524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc990152n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055156524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00059a003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00237a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055166521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00250a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055167025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cen-v074n034.p006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055360711"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00069a076", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055704099"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00081a069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055705244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00198a063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055713562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00242a020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055717014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0177915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055818938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja0177915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055818938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja983183m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055870784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la00018a030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056137463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la981729c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056170776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la981729c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056170776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8475384", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655745"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-01-06T00:00", 
    "description": "

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

", "id": "sg:patent.US-6673548-B2", "keywords": [ "nanoparticles", "oligonucleotides", "therefor", "invention", "method", "nucleic acid", "particle", "embodiment", "sequence", "portion", "detectable change", "color change", "hybridization", "composition", "kit", "conjugate", "Nanostructures", "nanofabrication" ], "name": "Nanoparticles having oligonucleotides attached thereto and uses therefor", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.16753.36", "type": "Organization" }, { "id": "https://www.grid.ac/institutes/grid.422095.8", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-6673548-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:28", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03133.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-6673548-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-6673548-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-6673548-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-6673548-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

159 TRIPLES      15 PREDICATES      64 URIs      26 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-6673548-B2 schema:about anzsrc-for:3021
2 schema:author Na822b7ba3bb146d6a820d22fe99617a9
3 schema:citation sg:pub.10.1038/350631a0
4 sg:pub.10.1038/382607a0
5 sg:pub.10.1038/382609a0
6 sg:pub.10.1038/ng1296-441
7 https://doi.org/10.1002/adma.19950070907
8 https://doi.org/10.1016/0003-2697(84)90518-9
9 https://doi.org/10.1016/0040-4039(96)01381-0
10 https://doi.org/10.1016/0092-8674(77)90182-9
11 https://doi.org/10.1021/ac00100a008
12 https://doi.org/10.1021/bc990152n
13 https://doi.org/10.1021/bi00059a003
14 https://doi.org/10.1021/bi00237a005
15 https://doi.org/10.1021/bi00250a005
16 https://doi.org/10.1021/cen-v074n034.p006
17 https://doi.org/10.1021/ja00069a076
18 https://doi.org/10.1021/ja00081a069
19 https://doi.org/10.1021/ja00198a063
20 https://doi.org/10.1021/ja00242a020
21 https://doi.org/10.1021/ja010437m
22 https://doi.org/10.1021/ja0177915
23 https://doi.org/10.1021/ja983183m
24 https://doi.org/10.1021/la00018a030
25 https://doi.org/10.1021/la950776r
26 https://doi.org/10.1021/la981729c
27 https://doi.org/10.1039/cc9960000555
28 https://doi.org/10.1093/nar/23.4.696
29 https://doi.org/10.1093/nar/30.7.1558
30 https://doi.org/10.1126/science.277.5329.1078
31 https://doi.org/10.1126/science.8475384
32 https://doi.org/10.1146/annurev.bb.23.060194.002545
33 https://doi.org/10.1146/annurev.bioeng.3.1.335
34 schema:datePublished 2004-01-06T00:00
35 schema:description <p>The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.</p>
36 schema:keywords Nanostructures
37 color change
38 composition
39 conjugate
40 detectable change
41 embodiment
42 hybridization
43 invention
44 kit
45 method
46 nanofabrication
47 nanoparticles
48 nucleic acid
49 oligonucleotides
50 particle
51 portion
52 sequence
53 therefor
54 schema:name Nanoparticles having oligonucleotides attached thereto and uses therefor
55 schema:recipient https://www.grid.ac/institutes/grid.16753.36
56 https://www.grid.ac/institutes/grid.422095.8
57 schema:sameAs https://app.dimensions.ai/details/patent/US-6673548-B2
58 schema:sdDatePublished 2019-04-18T10:28
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher N5b6009371a784651b6bbe569adbeb69f
61 sgo:license sg:explorer/license/
62 sgo:sdDataset patents
63 rdf:type sgo:Patent
64 N04a536dbb5e34025a47beac24e496ff5 rdf:first N70eca22534944e90987611411ed86fcf
65 rdf:rest N5acfb5ae9f6c4945bf4ffd4ae084211b
66 N1c49b804c09240b491ad3b18bcaedf70 schema:name Robert C. Mucic
67 rdf:type schema:Person
68 N2a27fe51d6b443d8b5f0e393f5582353 rdf:first N3df641032d014bddad9880490e58ed99
69 rdf:rest N417a2d54e8be4eccbb667aa727e19c42
70 N3df641032d014bddad9880490e58ed99 schema:name Robert L. Letsinger
71 rdf:type schema:Person
72 N417a2d54e8be4eccbb667aa727e19c42 rdf:first N1c49b804c09240b491ad3b18bcaedf70
73 rdf:rest N04a536dbb5e34025a47beac24e496ff5
74 N5acfb5ae9f6c4945bf4ffd4ae084211b rdf:first Nd4a05c07f270465c9f7d025377576d33
75 rdf:rest Nf96e35b4c9e44477bec7dded4c9d4949
76 N5b6009371a784651b6bbe569adbeb69f schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N70eca22534944e90987611411ed86fcf schema:name James J. Storhoff
79 rdf:type schema:Person
80 N9a3cc26bbcfc443c801b5301b8b7e90d schema:name Thomas A. Taton
81 rdf:type schema:Person
82 Na822b7ba3bb146d6a820d22fe99617a9 rdf:first Nc55f3fef691044569b685312241d5e81
83 rdf:rest N2a27fe51d6b443d8b5f0e393f5582353
84 Nc55f3fef691044569b685312241d5e81 schema:name Chad A. Mirkin
85 rdf:type schema:Person
86 Nd4a05c07f270465c9f7d025377576d33 schema:name Robert Elghanian
87 rdf:type schema:Person
88 Nf96e35b4c9e44477bec7dded4c9d4949 rdf:first N9a3cc26bbcfc443c801b5301b8b7e90d
89 rdf:rest rdf:nil
90 anzsrc-for:3021 schema:inDefinedTermSet anzsrc-for:
91 rdf:type schema:DefinedTerm
92 sg:pub.10.1038/350631a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034775610
93 https://doi.org/10.1038/350631a0
94 rdf:type schema:CreativeWork
95 sg:pub.10.1038/382607a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030717946
96 https://doi.org/10.1038/382607a0
97 rdf:type schema:CreativeWork
98 sg:pub.10.1038/382609a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013811778
99 https://doi.org/10.1038/382609a0
100 rdf:type schema:CreativeWork
101 sg:pub.10.1038/ng1296-441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051306917
102 https://doi.org/10.1038/ng1296-441
103 rdf:type schema:CreativeWork
104 https://doi.org/10.1002/adma.19950070907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017153621
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1016/0003-2697(84)90518-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034491884
107 rdf:type schema:CreativeWork
108 https://doi.org/10.1016/0040-4039(96)01381-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047699773
109 rdf:type schema:CreativeWork
110 https://doi.org/10.1016/0092-8674(77)90182-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015148835
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1021/ac00100a008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054971987
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1021/bc990152n schema:sameAs https://app.dimensions.ai/details/publication/pub.1055156524
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1021/bi00059a003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159174
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1021/bi00237a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055166521
119 rdf:type schema:CreativeWork
120 https://doi.org/10.1021/bi00250a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055167025
121 rdf:type schema:CreativeWork
122 https://doi.org/10.1021/cen-v074n034.p006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055360711
123 rdf:type schema:CreativeWork
124 https://doi.org/10.1021/ja00069a076 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055704099
125 rdf:type schema:CreativeWork
126 https://doi.org/10.1021/ja00081a069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055705244
127 rdf:type schema:CreativeWork
128 https://doi.org/10.1021/ja00198a063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055713562
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1021/ja00242a020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055717014
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1021/ja010437m schema:sameAs https://app.dimensions.ai/details/publication/pub.1041739689
133 rdf:type schema:CreativeWork
134 https://doi.org/10.1021/ja0177915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055818938
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1021/ja983183m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055870784
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1021/la00018a030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056137463
139 rdf:type schema:CreativeWork
140 https://doi.org/10.1021/la950776r schema:sameAs https://app.dimensions.ai/details/publication/pub.1000739633
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1021/la981729c schema:sameAs https://app.dimensions.ai/details/publication/pub.1056170776
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1039/cc9960000555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042820289
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1093/nar/23.4.696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051767673
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1093/nar/30.7.1558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000482521
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1126/science.277.5329.1078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010525136
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1126/science.8475384 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655745
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1146/annurev.bb.23.060194.002545 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011235010
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1146/annurev.bioeng.3.1.335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002076643
157 rdf:type schema:CreativeWork
158 https://www.grid.ac/institutes/grid.16753.36 schema:Organization
159 https://www.grid.ac/institutes/grid.422095.8 schema:Organization
 




Preview window. Press ESC to close (or click here)


...