Modified enzymes and their use for peptide synthesis


Ontology type: sgo:Patent     


Patent Info

DATE

2003-06-10T00:00

AUTHORS

J. Bryan Jones

ABSTRACT

The present invention relates to modified enzymes with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residue with a thiol side chain to form a modified enzyme, wherein the modified enzyme has high esterase and low amidase activity. Also, a method of producing the modified enzymes is provided. The present invention also relates to a method for using the modified enzymes in peptide synthesis. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "J. Bryan Jones", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1139/o80-086", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000593876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/1381-1177(96)00010-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001374731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c39860000417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003358095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(96)00057-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008026943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-894x(98)00415-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008185807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0960-894x(96)00467-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009529020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-894x(01)81221-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012182750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560050605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013659159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560050605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013659159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0922-338x(91)90079-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013982017"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0922-338x(91)90079-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013982017"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2960685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015594944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2960685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015594944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0040-4039(00)95261-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019461469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/cc9960000165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020149434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1630531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025333742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj1630531", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025333742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-9861(75)90141-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026289176"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(92)90662-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028424398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02983313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030254688", 
          "https://doi.org/10.1007/bf02983313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02983313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030254688", 
          "https://doi.org/10.1007/bf02983313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(88)80077-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032205731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00129339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032253607", 
          "https://doi.org/10.1007/bf00129339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00129339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032253607", 
          "https://doi.org/10.1007/bf00129339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0040-4020(01)89103-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033378553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(84)80133-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034450516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560020311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035372733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560020311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035372733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp9500730", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036352959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2710059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036389225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2710059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036389225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4838(89)90055-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036633220"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4838(89)90055-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036633220"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4838(92)90262-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036857572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-4838(92)90262-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036857572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01025550", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038234622", 
          "https://doi.org/10.1007/bf01025550"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(74)80110-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040343992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.21.12476", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041163248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4838(96)00147-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042673014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4838(96)00147-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042673014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pro.5560060803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045479148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-894x(01)81263-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046426562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1990.tb19484.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046916245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(98)00051-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048077428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c39870000533", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050740060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(71)90254-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052180251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(71)90254-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052180251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0040-4020(97)00562-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052980531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac970390g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055074057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac970390g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055074057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00020a006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055157478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00027a010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055157752"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00038a028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055158259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00064a034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00065a030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055159446"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00141a008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055162386"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00188a013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055164457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00231a007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055166273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00247a023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055166933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00302a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055169335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00367a018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055172255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00367a038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055172275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00567a028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055181065"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00675a019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055184901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9502093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055210619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9502093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055210619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9600759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9600759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi960823u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi960823u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9618629", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9618629", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055212630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9727951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055215101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9727951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055215101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00003a043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055698155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00094a005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055706341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00159a006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055710127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00169a043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055711106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00211a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055714525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00219a083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055715236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00223a042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055715576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00236a062", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055716583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00246a060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055717367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00246a064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055717371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00259a049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055718368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00272a052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055719321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00276a045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055719659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00297a054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055721309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00306a040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055721946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00965a060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055757626"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja952981z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055863723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja952981z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055863723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja954271z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055864349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja954271z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055864349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja964399w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055866632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja964399w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055866632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja970344y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055867155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja970344y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055867155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja970820k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055867369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja970820k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055867369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja980072e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055869309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf00014a037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055892149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jo00051a052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055963407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jo00364a021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055981531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1384130", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062478659"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-06-10T00:00", 
    "description": "

The present invention relates to modified enzymes with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residue with a thiol side chain to form a modified enzyme, wherein the modified enzyme has high esterase and low amidase activity. Also, a method of producing the modified enzymes is provided. The present invention also relates to a method for using the modified enzymes in peptide synthesis.

", "id": "sg:patent.US-6576454-B2", "keywords": [ "modified enzyme", "peptide synthesis", "invention", "amino acid residue", "enzyme", "cysteine residue", "hydrogen", "side chain", "wherein", "esterases", "method" ], "name": "Modified enzymes and their use for peptide synthesis", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.17063.33", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-6576454-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:28", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03124.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-6576454-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-6576454-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-6576454-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-6576454-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

282 TRIPLES      15 PREDICATES      107 URIs      19 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-6576454-B2 schema:about anzsrc-for:2581
2 schema:author N01428d9a97f64b0897adcc048b663cec
3 schema:citation sg:pub.10.1007/bf00129339
4 sg:pub.10.1007/bf01025550
5 sg:pub.10.1007/bf02983313
6 https://doi.org/10.1002/pro.5560020311
7 https://doi.org/10.1002/pro.5560050605
8 https://doi.org/10.1002/pro.5560060803
9 https://doi.org/10.1016/0003-9861(75)90141-1
10 https://doi.org/10.1016/0005-2744(71)90254-3
11 https://doi.org/10.1016/0014-5793(74)80110-9
12 https://doi.org/10.1016/0014-5793(84)80133-7
13 https://doi.org/10.1016/0022-2836(92)90662-4
14 https://doi.org/10.1016/0167-4838(89)90055-1
15 https://doi.org/10.1016/0167-4838(92)90262-c
16 https://doi.org/10.1016/0922-338x(91)90079-v
17 https://doi.org/10.1016/0960-894x(96)00467-2
18 https://doi.org/10.1016/1381-1177(96)00010-0
19 https://doi.org/10.1016/s0006-291x(88)80077-9
20 https://doi.org/10.1016/s0040-4020(01)89103-1
21 https://doi.org/10.1016/s0040-4020(97)00562-0
22 https://doi.org/10.1016/s0040-4039(00)95261-4
23 https://doi.org/10.1016/s0141-0229(96)00057-9
24 https://doi.org/10.1016/s0141-0229(98)00051-9
25 https://doi.org/10.1016/s0167-4838(96)00147-1
26 https://doi.org/10.1016/s0960-894x(01)81221-x
27 https://doi.org/10.1016/s0960-894x(01)81263-4
28 https://doi.org/10.1016/s0960-894x(98)00415-6
29 https://doi.org/10.1021/ac970390g
30 https://doi.org/10.1021/bi00020a006
31 https://doi.org/10.1021/bi00027a010
32 https://doi.org/10.1021/bi00038a028
33 https://doi.org/10.1021/bi00064a034
34 https://doi.org/10.1021/bi00065a030
35 https://doi.org/10.1021/bi00141a008
36 https://doi.org/10.1021/bi00188a013
37 https://doi.org/10.1021/bi00231a007
38 https://doi.org/10.1021/bi00247a023
39 https://doi.org/10.1021/bi00302a009
40 https://doi.org/10.1021/bi00367a018
41 https://doi.org/10.1021/bi00367a038
42 https://doi.org/10.1021/bi00567a028
43 https://doi.org/10.1021/bi00675a019
44 https://doi.org/10.1021/bi9502093
45 https://doi.org/10.1021/bi9600759
46 https://doi.org/10.1021/bi960823u
47 https://doi.org/10.1021/bi9618629
48 https://doi.org/10.1021/bi9727951
49 https://doi.org/10.1021/bp9500730
50 https://doi.org/10.1021/ja00003a043
51 https://doi.org/10.1021/ja00094a005
52 https://doi.org/10.1021/ja00159a006
53 https://doi.org/10.1021/ja00169a043
54 https://doi.org/10.1021/ja00211a004
55 https://doi.org/10.1021/ja00219a083
56 https://doi.org/10.1021/ja00223a042
57 https://doi.org/10.1021/ja00236a062
58 https://doi.org/10.1021/ja00246a060
59 https://doi.org/10.1021/ja00246a064
60 https://doi.org/10.1021/ja00259a049
61 https://doi.org/10.1021/ja00272a052
62 https://doi.org/10.1021/ja00276a045
63 https://doi.org/10.1021/ja00297a054
64 https://doi.org/10.1021/ja00306a040
65 https://doi.org/10.1021/ja00965a060
66 https://doi.org/10.1021/ja952981z
67 https://doi.org/10.1021/ja954271z
68 https://doi.org/10.1021/ja964399w
69 https://doi.org/10.1021/ja970344y
70 https://doi.org/10.1021/ja970820k
71 https://doi.org/10.1021/ja980072e
72 https://doi.org/10.1021/jf00014a037
73 https://doi.org/10.1021/jo00051a052
74 https://doi.org/10.1021/jo00364a021
75 https://doi.org/10.1039/c39860000417
76 https://doi.org/10.1039/c39870000533
77 https://doi.org/10.1039/cc9960000165
78 https://doi.org/10.1042/bj1630531
79 https://doi.org/10.1042/bj2710059
80 https://doi.org/10.1042/bj2960685
81 https://doi.org/10.1074/jbc.270.21.12476
82 https://doi.org/10.1111/j.1432-1033.1990.tb19484.x
83 https://doi.org/10.1126/science.1384130
84 https://doi.org/10.1139/o80-086
85 schema:datePublished 2003-06-10T00:00
86 schema:description <p>The present invention relates to modified enzymes with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residue with a thiol side chain to form a modified enzyme, wherein the modified enzyme has high esterase and low amidase activity. Also, a method of producing the modified enzymes is provided. The present invention also relates to a method for using the modified enzymes in peptide synthesis.</p>
87 schema:keywords amino acid residue
88 cysteine residue
89 enzyme
90 esterases
91 hydrogen
92 invention
93 method
94 modified enzyme
95 peptide synthesis
96 side chain
97 wherein
98 schema:name Modified enzymes and their use for peptide synthesis
99 schema:recipient https://www.grid.ac/institutes/grid.17063.33
100 schema:sameAs https://app.dimensions.ai/details/patent/US-6576454-B2
101 schema:sdDatePublished 2019-04-18T10:28
102 schema:sdLicense https://scigraph.springernature.com/explorer/license/
103 schema:sdPublisher N6c1c4ab017db4604bd749b5a488457b0
104 sgo:license sg:explorer/license/
105 sgo:sdDataset patents
106 rdf:type sgo:Patent
107 N01428d9a97f64b0897adcc048b663cec rdf:first Ne1442de454a14d35a0391b835a6e7b8c
108 rdf:rest rdf:nil
109 N6c1c4ab017db4604bd749b5a488457b0 schema:name Springer Nature - SN SciGraph project
110 rdf:type schema:Organization
111 Ne1442de454a14d35a0391b835a6e7b8c schema:name J. Bryan Jones
112 rdf:type schema:Person
113 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
114 rdf:type schema:DefinedTerm
115 sg:pub.10.1007/bf00129339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032253607
116 https://doi.org/10.1007/bf00129339
117 rdf:type schema:CreativeWork
118 sg:pub.10.1007/bf01025550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038234622
119 https://doi.org/10.1007/bf01025550
120 rdf:type schema:CreativeWork
121 sg:pub.10.1007/bf02983313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030254688
122 https://doi.org/10.1007/bf02983313
123 rdf:type schema:CreativeWork
124 https://doi.org/10.1002/pro.5560020311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035372733
125 rdf:type schema:CreativeWork
126 https://doi.org/10.1002/pro.5560050605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013659159
127 rdf:type schema:CreativeWork
128 https://doi.org/10.1002/pro.5560060803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045479148
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1016/0003-9861(75)90141-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026289176
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1016/0005-2744(71)90254-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052180251
133 rdf:type schema:CreativeWork
134 https://doi.org/10.1016/0014-5793(74)80110-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040343992
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1016/0014-5793(84)80133-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034450516
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1016/0022-2836(92)90662-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028424398
139 rdf:type schema:CreativeWork
140 https://doi.org/10.1016/0167-4838(89)90055-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036633220
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1016/0167-4838(92)90262-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1036857572
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1016/0922-338x(91)90079-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1013982017
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/0960-894x(96)00467-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009529020
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1016/1381-1177(96)00010-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001374731
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1016/s0006-291x(88)80077-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032205731
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1016/s0040-4020(01)89103-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033378553
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/s0040-4020(97)00562-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052980531
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1016/s0040-4039(00)95261-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019461469
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/s0141-0229(96)00057-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008026943
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/s0141-0229(98)00051-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048077428
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/s0167-4838(96)00147-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042673014
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/s0960-894x(01)81221-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012182750
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/s0960-894x(01)81263-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046426562
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/s0960-894x(98)00415-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008185807
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1021/ac970390g schema:sameAs https://app.dimensions.ai/details/publication/pub.1055074057
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1021/bi00020a006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055157478
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1021/bi00027a010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055157752
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1021/bi00038a028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055158259
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1021/bi00064a034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159403
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1021/bi00065a030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055159446
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1021/bi00141a008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055162386
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1021/bi00188a013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055164457
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1021/bi00231a007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055166273
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1021/bi00247a023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055166933
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1021/bi00302a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055169335
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1021/bi00367a018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055172255
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1021/bi00367a038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055172275
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1021/bi00567a028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055181065
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1021/bi00675a019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055184901
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1021/bi9502093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055210619
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1021/bi9600759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211578
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1021/bi960823u schema:sameAs https://app.dimensions.ai/details/publication/pub.1055212037
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1021/bi9618629 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055212630
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1021/bi9727951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055215101
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1021/bp9500730 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036352959
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1021/ja00003a043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055698155
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1021/ja00094a005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055706341
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1021/ja00159a006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055710127
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1021/ja00169a043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055711106
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1021/ja00211a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055714525
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1021/ja00219a083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055715236
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1021/ja00223a042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055715576
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1021/ja00236a062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055716583
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1021/ja00246a060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055717367
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1021/ja00246a064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055717371
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1021/ja00259a049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055718368
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/ja00272a052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055719321
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/ja00276a045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055719659
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1021/ja00297a054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055721309
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1021/ja00306a040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055721946
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1021/ja00965a060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055757626
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1021/ja952981z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055863723
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1021/ja954271z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055864349
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1021/ja964399w schema:sameAs https://app.dimensions.ai/details/publication/pub.1055866632
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1021/ja970344y schema:sameAs https://app.dimensions.ai/details/publication/pub.1055867155
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1021/ja970820k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055867369
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1021/ja980072e schema:sameAs https://app.dimensions.ai/details/publication/pub.1055869309
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/jf00014a037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055892149
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/jo00051a052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055963407
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1021/jo00364a021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055981531
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1039/c39860000417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003358095
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1039/c39870000533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050740060
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1039/cc9960000165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020149434
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1042/bj1630531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025333742
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1042/bj2710059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036389225
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1042/bj2960685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015594944
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1074/jbc.270.21.12476 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041163248
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1111/j.1432-1033.1990.tb19484.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046916245
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.1384130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062478659
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1139/o80-086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000593876
281 rdf:type schema:CreativeWork
282 https://www.grid.ac/institutes/grid.17063.33 schema:Organization
 




Preview window. Press ESC to close (or click here)


...