Useful mutations of bacterial alkaline protease


Ontology type: sgo:Patent     


Patent Info

DATE

2003-01-14T00:00

AUTHORS

Sven Hastrup , Sven Branner , Fanny Norris , Steffen Bjørn Petersen , Leif Nørskov-Lauridsen , Villy Johannes Jensen , Dorrit Aaslyng

ABSTRACT

The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2581", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Sven Hastrup", 
        "type": "Person"
      }, 
      {
        "name": "Sven Branner", 
        "type": "Person"
      }, 
      {
        "name": "Fanny Norris", 
        "type": "Person"
      }, 
      {
        "name": "Steffen Bj\u00f8rn Petersen", 
        "type": "Person"
      }, 
      {
        "name": "Leif N\u00f8rskov-Lauridsen", 
        "type": "Person"
      }, 
      {
        "name": "Villy Johannes Jensen", 
        "type": "Person"
      }, 
      {
        "name": "Dorrit Aaslyng", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1098/rsta.1986.0051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000492223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(71)90107-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002775441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(84)90150-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003269210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.15.5167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010601977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rstb.1970.0014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014061789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.11.3743", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014828431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1972.tb01754.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019632595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00215583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020119678", 
          "https://doi.org/10.1007/bf00215583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00215583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020119678", 
          "https://doi.org/10.1007/bf00215583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/330086a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032013914", 
          "https://doi.org/10.1038/330086a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/221235a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041115847", 
          "https://doi.org/10.1038/221235a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/221235a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041115847", 
          "https://doi.org/10.1038/221235a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(88)90372-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041413428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/318375a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046764988", 
          "https://doi.org/10.1038/318375a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(87)90360-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046966310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-9861(83)90592-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048399032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.340010406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052670768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.340010406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052670768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00382a002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055173001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00662a012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055184442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00763a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055187777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/11.22.7911", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059931649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/1.4.319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059980339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/sqb.1972.036.01.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060404154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.233.4764.659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062532863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1986.tb04286.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079797862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb01968.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1081710957"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-01-14T00:00", 
    "description": "

The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from Bacillus lentus variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.

", "id": "sg:patent.US-6506589-B1", "keywords": [ "mutation", "alkaline", "invention", "subtilisin", "chemical characteristic", "nucleic acid", "embodiment", "mutant enzyme", "chemical property", "increased stability", "oxidation", "proteolytic activity", "amino acid", "Base Sequence", "protease", "bacillus", "variant", "gene" ], "name": "Useful mutations of bacterial alkaline protease", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.10582.3e", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-6506589-B1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:28", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_03118.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-6506589-B1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-6506589-B1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-6506589-B1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-6506589-B1'


 

This table displays all metadata directly associated to this object as RDF triples.

143 TRIPLES      15 PREDICATES      57 URIs      26 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-6506589-B1 schema:about anzsrc-for:2581
2 anzsrc-for:2620
3 schema:author N023cdc52a9e54f99aea6b4727079c1fd
4 schema:citation sg:pub.10.1007/bf00215583
5 sg:pub.10.1038/221235a0
6 sg:pub.10.1038/318375a0
7 sg:pub.10.1038/330086a0
8 https://doi.org/10.1002/j.1460-2075.1984.tb01968.x
9 https://doi.org/10.1002/j.1460-2075.1986.tb04286.x
10 https://doi.org/10.1002/prot.340010406
11 https://doi.org/10.1016/0003-9861(83)90592-1
12 https://doi.org/10.1016/0006-291x(71)90107-0
13 https://doi.org/10.1016/0022-2836(84)90150-5
14 https://doi.org/10.1016/0022-2836(87)90360-3
15 https://doi.org/10.1016/0022-2836(88)90372-5
16 https://doi.org/10.1021/bi00382a002
17 https://doi.org/10.1021/bi00662a012
18 https://doi.org/10.1021/bi00763a009
19 https://doi.org/10.1073/pnas.83.11.3743
20 https://doi.org/10.1073/pnas.84.15.5167
21 https://doi.org/10.1093/nar/11.22.7911
22 https://doi.org/10.1093/protein/1.4.319
23 https://doi.org/10.1098/rsta.1986.0051
24 https://doi.org/10.1098/rstb.1970.0014
25 https://doi.org/10.1101/sqb.1972.036.01.017
26 https://doi.org/10.1111/j.1432-1033.1972.tb01754.x
27 https://doi.org/10.1126/science.233.4764.659
28 schema:datePublished 2003-01-14T00:00
29 schema:description <p>The present invention relates to mutations of the subtilisin gene, some of which result in changes in the chemical characteristics of subtilisin enzyme. Mutations are created at specific nucleic acids of the subtilisin gene and, in various specific embodiments, the mutant enzymes possess altered chemical properties including, but not limited to, increased stability to oxidation, augmented proteolytic activity, and improved washability. The present invention also relates, but is not limited to, the amino acid and DNA sequences for two proteases derived from <i>Bacillus lentus </i>variants, subtilisin 147 and subtilisin 309, as well as mutations of these genes and the corresponding mutant enzymes.</p>
30 schema:keywords Base Sequence
31 alkaline
32 amino acid
33 bacillus
34 chemical characteristic
35 chemical property
36 embodiment
37 gene
38 increased stability
39 invention
40 mutant enzyme
41 mutation
42 nucleic acid
43 oxidation
44 protease
45 proteolytic activity
46 subtilisin
47 variant
48 schema:name Useful mutations of bacterial alkaline protease
49 schema:recipient https://www.grid.ac/institutes/grid.10582.3e
50 schema:sameAs https://app.dimensions.ai/details/patent/US-6506589-B1
51 schema:sdDatePublished 2019-04-18T10:28
52 schema:sdLicense https://scigraph.springernature.com/explorer/license/
53 schema:sdPublisher Nb1e964aaa5dd46a298ec3d969a42b622
54 sgo:license sg:explorer/license/
55 sgo:sdDataset patents
56 rdf:type sgo:Patent
57 N023cdc52a9e54f99aea6b4727079c1fd rdf:first N06487489eedb4a8b986e19c785fc9d47
58 rdf:rest N1257b2bcd2564249be068e7f6f62a8ec
59 N06487489eedb4a8b986e19c785fc9d47 schema:name Sven Hastrup
60 rdf:type schema:Person
61 N0c7d40d9637d4e38a92005ddeb74b4ec rdf:first Nef8ca7fd299e45d2adf87a66fd36b17f
62 rdf:rest N0e2147983e0f4224b62eb081a64e21a5
63 N0e2147983e0f4224b62eb081a64e21a5 rdf:first Ne1ace9e1e61545cabcc518c68cb42eb6
64 rdf:rest rdf:nil
65 N1257b2bcd2564249be068e7f6f62a8ec rdf:first N959bdbdb799f401084ebc2946c5a3e2f
66 rdf:rest N9b93c7fd7e254af993dd94fb5a750157
67 N26a1bdf1e2a4436799d90ac7b5eed86d schema:name Leif Nørskov-Lauridsen
68 rdf:type schema:Person
69 N60b58ed90d454ea39ec3273dec3540d6 rdf:first N26a1bdf1e2a4436799d90ac7b5eed86d
70 rdf:rest N0c7d40d9637d4e38a92005ddeb74b4ec
71 N959bdbdb799f401084ebc2946c5a3e2f schema:name Sven Branner
72 rdf:type schema:Person
73 N9b93c7fd7e254af993dd94fb5a750157 rdf:first Nba5a5385ac0441ac86f25eef81870b32
74 rdf:rest Nb9eab61b51324838b24eca731ed79750
75 N9ce0c091ce8a47edba480db5aca626dd schema:name Steffen Bjørn Petersen
76 rdf:type schema:Person
77 Nb1e964aaa5dd46a298ec3d969a42b622 schema:name Springer Nature - SN SciGraph project
78 rdf:type schema:Organization
79 Nb9eab61b51324838b24eca731ed79750 rdf:first N9ce0c091ce8a47edba480db5aca626dd
80 rdf:rest N60b58ed90d454ea39ec3273dec3540d6
81 Nba5a5385ac0441ac86f25eef81870b32 schema:name Fanny Norris
82 rdf:type schema:Person
83 Ne1ace9e1e61545cabcc518c68cb42eb6 schema:name Dorrit Aaslyng
84 rdf:type schema:Person
85 Nef8ca7fd299e45d2adf87a66fd36b17f schema:name Villy Johannes Jensen
86 rdf:type schema:Person
87 anzsrc-for:2581 schema:inDefinedTermSet anzsrc-for:
88 rdf:type schema:DefinedTerm
89 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
90 rdf:type schema:DefinedTerm
91 sg:pub.10.1007/bf00215583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020119678
92 https://doi.org/10.1007/bf00215583
93 rdf:type schema:CreativeWork
94 sg:pub.10.1038/221235a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041115847
95 https://doi.org/10.1038/221235a0
96 rdf:type schema:CreativeWork
97 sg:pub.10.1038/318375a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046764988
98 https://doi.org/10.1038/318375a0
99 rdf:type schema:CreativeWork
100 sg:pub.10.1038/330086a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032013914
101 https://doi.org/10.1038/330086a0
102 rdf:type schema:CreativeWork
103 https://doi.org/10.1002/j.1460-2075.1984.tb01968.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1081710957
104 rdf:type schema:CreativeWork
105 https://doi.org/10.1002/j.1460-2075.1986.tb04286.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079797862
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1002/prot.340010406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052670768
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/0003-9861(83)90592-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048399032
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/0006-291x(71)90107-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002775441
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1016/0022-2836(84)90150-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003269210
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/0022-2836(87)90360-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046966310
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1016/0022-2836(88)90372-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041413428
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1021/bi00382a002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055173001
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1021/bi00662a012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055184442
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1021/bi00763a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055187777
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1073/pnas.83.11.3743 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014828431
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1073/pnas.84.15.5167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010601977
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1093/nar/11.22.7911 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059931649
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1093/protein/1.4.319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059980339
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1098/rsta.1986.0051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000492223
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1098/rstb.1970.0014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014061789
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1101/sqb.1972.036.01.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060404154
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1111/j.1432-1033.1972.tb01754.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019632595
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1126/science.233.4764.659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062532863
142 rdf:type schema:CreativeWork
143 https://www.grid.ac/institutes/grid.10582.3e schema:Organization
 




Preview window. Press ESC to close (or click here)


...