Method for transformation of cotton and organogenic regeneration


Ontology type: sgo:Patent     


Patent Info

DATE

2002-11-12T00:00

AUTHORS

REICHERT NANCY A , LIM TEONG-KWEE , YOUNG MARGARET M

ABSTRACT

Protocols for organogenic regeneration of cotton are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO3. These explants, prior to shoot initiation, may be transformed with exogenous DNA, either through inoculation with an Agorbacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface. More... »

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