Reduction of nonspecific hybridization by using novel base-pairing schemes


Ontology type: sgo:Patent     


Patent Info

DATE

2001-05-15T00:00

AUTHORS

Mark L. Collins , Thomas Horn , Patrick J Sheridan , Brian D. Warner , Michael S. Urdea

ABSTRACT

Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2′-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery. More... »

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