Mutant proteolytic enzymes and method of production


Ontology type: sgo:Patent     


Patent Info

DATE

2000-10-24T00:00

AUTHORS

Teresa Christianson , Dean Goddette , Beth Frances Ladin , Maria R. Lau , Christian Paech , Robert B. Reynolds , Charles R. Wilson , Shiow-Shong Yang

ABSTRACT

Mutant B. lentus DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the B. lentus DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease. More... »

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