Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Ronald C. Lundquist , David A. Walters , Julie A. Kirihara

ABSTRACT

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile lants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen. More... »

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