Insulin-secreting cell lines, methods of production and use


Ontology type: sgo:Patent     


Patent Info

DATE

1999-05-11T00:00

AUTHORS

Maryam Asfari , Paul Czernichow

ABSTRACT

The invention relates to the field of biology and, in particular, to the field of cellular biology. The invention concerns a novel glucose-sensitive cell line designated β cell line (INS-I) expressing glucokinase and the glucose carrier Glut 2 at levels comparable with those of normal β cells but which is, in addition, incapable of IGF-II expression-dependent proliferation because of genetic manipulation. The invention also concerns a method for the production of said novel cell line, its aggregation in the form of a pseudoislet, its immobilization in a biocompatible hydrogel and its hardening by means of a hardening solution. Application in insulin-secreting β-cell transplants. More... »

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