Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A


Ontology type: sgo:Patent     


Patent Info

DATE

1998-08-18T00:00

AUTHORS

David B. Powers , Stephen Anderson

ABSTRACT

Mutants of 2,5-diketo-D-gluconic acid reductase A an enzyme used to produce 2-keto-L-gluconic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate. More... »

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